Application
Western blot
Sample
Human Cell lysate - whole cell (Hela, HepG2)
Gel Running Conditions
Reduced Denaturing (4-12% BisTris Gel)
Loading amount
10 µg
Specification
Hela, HepG2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: ECL Rabbit IgG, HRP-linked whole Ab from donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 45 kDa
Additional data
Additional Notes
1-Hela cell lysate
2-HepG2 cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 18 2019