Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (Brain sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris EDTA pH 9.0
Permeabilization
No
Specification
Brain sections
Blocking step
BSA + Milk + Serum as blocking agent for 20 minute(s) · Concentration: 2.0% · Temperature: 27°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/50
Secondary antibody
Dilution
1/750
Name
Non-Abcam antibody was used: ThermoFischer A-11008 - Goat anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
Specimen: chicken brain sections
Fixative: Formalin in Millonig buffer (48 hours)
Antigen retrieval in Pascal(R) chamber
Counterstainings: Evans blue (Red) and DAPI (Blue)
Image acquisition: Zeiss LSM 710 confocal microscope
Obs: This antibody did not work either in our specimens and in our positive controls (mouse brain tissues).
Abcam response
The end user has tested this antibody under Abtrial offer (https://www.abcam.com/content/abtrial-program-test-risk-free).
The immunogenic extracellular domain sequence of human doesn't show a good homology therefore, this product may not be a good choice for Chicken samples.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Marcelo Pelajo Machado
Verified customer
投稿 Jul 21 2017