Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Murine cells)
Specification
Murine cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 65 kDa Non-specific: 45, 55, 120 kDa
Exposure
10 minute(s) and 0 second(s)
Positive control
Lane 4, HeLa whole cell lysate
Additional data
Additional Notes
Lane 1 = primary mouse cerebellar granule neuron precursors. Lanes 2, 3 = mouse medulloblastoma cell lines. Lane 4 = Hela cells (human).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 10 2014