Anti-FANCL 抗体 (ab272618)
Key features and details
- Rabbit polyclonal to FANCL
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FANCL antibody -
製品の詳細
Rabbit polyclonal to FANCL -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human FANCL aa 50-200.
Database link: Q9NW38 -
ポジティブ・コントロール
- WB: NTERA-2. IHC-P: Human duodenum tissue. ICC/IF: U-2 OS.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272618の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ターゲット情報
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機能
Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth. -
パスウェイ
Protein modification; protein ubiquitination. -
関連疾患
Fanconi anemia complementation group L -
配列類似性
Contains 1 RING-type zinc finger. -
ドメイン
The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2. -
翻訳後修飾
The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 55120 Human
- Omim: 608111 Human
- SwissProt: Q9NW38 Human
- Unigene: 631890 Human
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別名
- E3 ubiquitin-protein ligase FANCL antibody
- FAAP43 antibody
- Fancl antibody
see all
画像
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Anti-FANCL antibody (ab272618) at 0.4 µg/ml + NTERA-2
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Paraffin-embedded formalin-fixed Human duodenum tissue stained for FANCL using ab272618 at 1/50 in immunohistochemical analysis.
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PFA fixed, Triton X-100 permeablized human cell line U-2 OS labeling FANCL (green) using ab272618at 2 ug/ml in ICC/IF analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272618 は論文での使用が確認できていません。