Anti-FANCL 抗体 (ab272618)

Submit a review Submit a question
Western blot - Anti-FANCL antibody (ab272618)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCL antibody (ab272618)
  • Immunocytochemistry/ Immunofluorescence - Anti-FANCL antibody (ab272618)

Key features and details

  • Rabbit polyclonal to FANCL
  • Suitable for: ICC/IF, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-FANCL antibody

  • 製品の詳細

    Rabbit polyclonal to FANCL

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment corresponding to Human FANCL aa 50-200.
    Database link: Q9NW38

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: NTERA-2. IHC-P: Human duodenum tissue. ICC/IF: U-2 OS.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC/IF
Human
IHC-P
Human
WB
Human
アプリケーション Abreviews 特記事項
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.
特記事項
ICC/IF
Use a concentration of 0.25 - 2 µg/ml.
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 0.04 - 0.4 µg/ml.

ターゲット情報

  • 機能

    Ubiquitin ligase protein that mediates monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.

  • パスウェイ

    Protein modification; protein ubiquitination.

  • 関連疾患

    Fanconi anemia complementation group L

  • 配列類似性

    Contains 1 RING-type zinc finger.

  • ドメイン

    The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.

  • 翻訳後修飾

    The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.

  • 細胞内局在

    Cytoplasm. Nucleus.
  • Information by UniProt

  • 参照データベース

    • 別名

      • E3 ubiquitin-protein ligase FANCL antibody
      • FAAP43 antibody
      • Fancl antibody
      • FANCL_HUMAN antibody
      • Fanconi anemia complementation group L antibody
      • Fanconi anemia group L protein antibody
      • Fanconi anemia-associated polypeptide of 43 kDa antibody
      • FLJ10335 antibody
      • PHD finger protein 9 antibody
      • PHF9 antibody
      • POG antibody
      • Ubiquitin ligase protein FANCL antibody
      see all

    画像

    • Western blot - Anti-FANCL antibody (ab272618)
      Western blot - Anti-FANCL antibody (ab272618)
      Anti-FANCL antibody (ab272618) at 0.4 µg/ml + NTERA-2
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCL antibody (ab272618)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FANCL antibody (ab272618)

      Paraffin-embedded formalin-fixed Human duodenum tissue stained for FANCL using ab272618 at 1/50 in immunohistochemical analysis.

    • Immunocytochemistry/ Immunofluorescence - Anti-FANCL antibody (ab272618)
      Immunocytochemistry/ Immunofluorescence - Anti-FANCL antibody (ab272618)

      PFA fixed, Triton X-100 permeablized human cell line U-2 OS labeling FANCL (green) using ab272618at 2 ug/ml in ICC/IF analysis.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272618 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272618 は論文での使用が確認できていません。

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    There are currently no Customer reviews or Questions for ab272618.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com