Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4-12 Bis tris)
Sample
Human Cell lysate - whole cell (293T)
Specification
293T
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C · Diluent: PBS tween 5% milk
Dilution
1/2500
Secondary antibody
Name
Non-Abcam antibody was used: Anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL prime
Bands
Specific: 150 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Additional Notes
Blocking was made overnight at 4¯, rocking
1st Ab at 18¯C 1h rocking.
2nd antibody 18¯C 1h rocking.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 31 2014