製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to FANCC
  • 由来種
    Rabbit
  • 特異性
    Detects a band at 60kDa in Hela cell lysate corresponding to FANCC.
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse
  • 免疫原

    Synthetic peptide:

    CLINKEPQNSGSKLNS

    , corresponding to amino acids 96 - 112 of Human FANCC.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.878% Sodium chloride
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).

ターゲット情報

  • 機能
    DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1.
  • 組織特異性
    Ubiquitous.
  • 関連疾患
    Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair.
  • 発生段階
    Expression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level).
  • 細胞内局在
    Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bA80I15.1 antibody
    • FA 3 antibody
    • FA3 antibody
    • FAC antibody
    • FACC antibody
    • FANCC antibody
    • FANCC_HUMAN antibody
    • Fanconi anemia complementation group C antibody
    • Fanconi anemia complementation group C protein antibody
    • Fanconi anemia group C protein antibody
    • Fanconi pancytopenia type 3 antibody
    • FLJ14675 antibody
    • Protein FACC antibody
    see all

画像

  • Anti-FANCC antibody (ab5065) at 1/1500 dilution + HeLa whole cell lysate at 35 µg

    Secondary
    IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

    Predicted band size: 63 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.



    Primary antibody incubated with PBS supplemented with 1% normal goat serum and 0.1% milk.
  • ICC/IF image of ab5065 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Lu WT  et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB ; Human . Read more (PubMed: 21926477) »
  • Palagyi A  et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer 9:127 (2010). WB ; Human . Read more (PubMed: 20509860) »
See all 2 Publications for this product

レビューと Q&A

Answer

Thank you for contacting us.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録