Anti-FANCC 抗体 (ab5065)
Key features and details
- Rabbit polyclonal to FANCC
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FANCC antibody
FANCC 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FANCC -
由来種
Rabbit -
特異性
Detects a band at 60kDa in Hela cell lysate corresponding to FANCC. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Human
非交差種: Mouse -
免疫原
Synthetic peptide:
CLINKEPQNSGSKLNS
, corresponding to amino acids 96 - 112 of Human FANCC.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.878% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab5065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa). |
ターゲット情報
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機能
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be implicated in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. Upon IFNG induction, may facilitate STAT1 activation by recruiting STAT1 to IFNGR1. -
組織特異性
Ubiquitous. -
関連疾患
Defects in FANCC are the cause of Fanconi anemia complementation group C (FANCC) [MIM:227645]. A disorder affecting all bone marrow elements and resulting in anemia, leukopenia and thrombopenia. It is associated with cardiac, renal and limb malformations, dermal pigmentary changes, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage) and defective DNA repair. -
発生段階
Expression increases during S phase, is maximal at the G2/M transition, and declines during M phase (at protein level). -
細胞内局在
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 2176 Human
- Omim: 613899 Human
- SwissProt: Q00597 Human
- Unigene: 494529 Human
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別名
- bA80I15.1 antibody
- FA 3 antibody
- FA3 antibody
see all
画像
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Western blot - Anti-FANCC antibody (ab5065)Anti-FANCC antibody (ab5065) at 1/1500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Predicted band size: 63 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 33 kDa. We are unsure as to the identity of these extra bands.
Primary antibody incubated with PBS supplemented with 1% normal goat serum and 0.1% milk. -
Immunocytochemistry/ Immunofluorescence - Anti-FANCC antibody (ab5065)ICC/IF image of ab5065 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5065, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
参考文献 (2)
ab5065 は 2 報の論文で使用されています。
- Lu WT et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB ; Human . PubMed: 21926477
- Palagyi A et al. Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents. Mol Cancer 9:127 (2010). WB ; Human . PubMed: 20509860
