Anti-FANCA/FAA 抗体 [EPR16519] (ab201457)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16519] to FANCA/FAA
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-FANCA/FAA antibody [EPR16519]
FANCA/FAA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16519] to FANCA/FAA -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HeLa, HEK-293, A431, A549, HAP1 and Jurkat whole cell lysates; Human colon lysate. IP: HeLa whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16519 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab201457の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/30.
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WB |
1/1000. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa).
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特記事項 |
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IP
1/30. |
WB
1/1000. Detects a band of approximately 163 kDa (predicted molecular weight: 163 kDa). |
ターゲット情報
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機能
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. -
関連疾患
Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H. -
細胞内局在
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 2175 Human
- Omim: 607139 Human
- SwissProt: O15360 Human
- Unigene: 290154 Human
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別名
- FA 1 antibody
- FA antibody
- FA H antibody
see all
画像
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All lanes : Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : FANCA knockout A549 cell lysate
Lane 3 : Wild-type HAP1 cell lysate
Lane 4 : FANCA knockout HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 163 kDa
Observed band size: 163 kDaWestern blot: Anti-FANCA antibody [EPR16519] (ab201457) staining at 1/1000 dilution, shown in green; Mouse anti-CANX [CANX/1543] (ab238078) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab201457 was shown to bind specifically to FANCA. A band was observed at 163 kDa in wild-type A549 cell lysates with no signal observed at this size in FANCA knockout cell line. To generate this image, wild-type and FANCA knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1% Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: FANCA/FAA beta knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab201457 observed at 163 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab201457 was shown to specifically react with FANCA/FAA beta when FANCA/FAA beta knockout samples were used. Wild-type and FANCA/FAA beta knockout samples were subjected to SDS-PAGE. ab201457 and ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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All lanes : Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/10000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 4 : A431 (Human epidermoid carcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 163 kDa
Observed band size: 163 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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Anti-FANCA/FAA antibody [EPR16519] (ab201457) at 1/1000 dilution + Human colon lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 163 kDa
Observed band size: 163 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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FANCA/FAA was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab201457 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201457 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1500 dilution.
Lane 1: HeLa whole cell lysate 10 µg (Input). Lane 2: ab201457 IP in HeLa whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201457 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (3)
ab201457 は 3 報の論文で使用されています。
- Kalev P et al. MAT2A Inhibition Blocks the Growth of MTAP-Deleted Cancer Cells by Reducing PRMT5-Dependent mRNA Splicing and Inducing DNA Damage. Cancer Cell 39:209-224.e11 (2021). PubMed: 33450196
- Hambarde S et al. EXO5-DNA structure and BLM interactions direct DNA resection critical for ATR-dependent replication restart. Mol Cell 81:2989-3006.e9 (2021). PubMed: 34197737
- Zhong J et al. Acetylation of hMOF Modulates H4K16ac to Regulate DNA Repair Genes in Response to Oxidative Stress. Int J Biol Sci 13:923-934 (2017). PubMed: 28808424