Anti-FANCA/FAA 抗体 (ab5063)
Key features and details
- Rabbit polyclonal to FANCA/FAA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-FANCA/FAA antibody
FANCA/FAA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to FANCA/FAA -
由来種
Rabbit -
特異性
Detects a band at 130kDa in HeLa cell lysate that we believe corresponds to FANCA/FAA.
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human FANCA/FAA aa 995-1009 conjugated to keyhole limpet haemocyanin.
Sequence:SRSYDHSENSDLVFG-C
Database link: O15360 -
特記事項
This product was previously labelled as FANCA
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab5063 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/2000. Detects a band of approximately 130 kDa (predicted molecular weight: 163 kDa). |
ターゲット情報
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機能
DNA repair protein that may operate in a postreplication repair or a cell cycle checkpoint function. May be involved in interstrand DNA cross-link repair and in the maintenance of normal chromosome stability. -
関連疾患
Defects in FANCA are a cause of Fanconi anemia (FA) [MIM:227650]. FA is a genetically heterogeneous, autosomal recessive disorder characterized by progressive pancytopenia, a diverse assortment of congenital malformations, and a predisposition to the development of malignancies. At the cellular level it is associated with hypersensitivity to DNA-damaging agents, chromosomal instability (increased chromosome breakage), and defective DNA repair. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation is required for the formation of the nuclear complex. Not phosphorylated in cells derived from groups A, B, C, E, F, G, and H. -
細胞内局在
Nucleus. Cytoplasm. The major form is nuclear. The minor form is cytoplasmic. - Information by UniProt
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参照データベース
- Entrez Gene: 2175 Human
- Omim: 607139 Human
- SwissProt: O15360 Human
- Unigene: 290154 Human
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別名
- FA 1 antibody
- FA antibody
- FA H antibody
see all
画像
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Western blot - Anti-FANCA/FAA antibody (ab5063)Anti-FANCA/FAA antibody (ab5063) at 1/1500 dilution + HeLa whole cell lysate at 35 µg
Secondary
IRDye 800 conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 163 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
参考文献 (10)
ab5063 は 10 報の論文で使用されています。
- Román-Rodríguez FJ et al. NHEJ-Mediated Repair of CRISPR-Cas9-Induced DNA Breaks Efficiently Corrects Mutations in HSPCs from Patients with Fanconi Anemia. Cell Stem Cell 25:607-621.e7 (2019). PubMed: 31543367
- Abdul-Sater Z et al. FANCA safeguards interphase and mitosis during hematopoiesis in vivo. Exp Hematol 43:1031-1046.e12 (2015). IF . PubMed: 26366677
- Vanuytsel K et al. FANCA knockout in human embryonic stem cells causes a severe growth disadvantage. Stem Cell Res 13:240-50 (2014). WB . PubMed: 25108529
- Rio P et al. Targeted gene therapy and cell reprogramming in Fanconi anemia. EMBO Mol Med 6:835-48 (2014). WB ; Human . PubMed: 24859981
- Shen C et al. Regulation of FANCD2 by the mTOR pathway contributes to the resistance of cancer cells to DNA double-strand breaks. Cancer Res 73:3393-401 (2013). PubMed: 23633493
- Meier D & Schindler D Fanconi anemia core complex gene promoters harbor conserved transcription regulatory elements. PLoS One 6:e22911 (2011). WB . PubMed: 21826217
- Lu WT et al. The Fanconi anemia pathway is downregulated upon macrophage differentiation through two distinct mechanisms. Cell Cycle 10: (2011). WB ; Human . PubMed: 21926477
- Tulpule A et al. Knockdown of Fanconi anemia genes in human embryonic stem cells reveals early developmental defects in the hematopoietic lineage. Blood 115:3453-62 (2010). WB ; Human . PubMed: 20089964
- Mattera L et al. The E1A-associated p400 protein modulates cell fate decisions by the regulation of ROS homeostasis. PLoS Genet 6:e1000983 (2010). WB ; Human . PubMed: 20548951
- Wilson JB et al. FANCG promotes formation of a newly identified protein complex containing BRCA2, FANCD2 and XRCC3. Oncogene 27:3641-52 (2008). PubMed: 18212739
