遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-FAK 抗体 [EP695Y] - BSA and Azide free (ab271836)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)
  • Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP695Y] to FAK - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Rat, Cow, Human

製品の概要

  • 製品名

    Anti-FAK antibody [EP695Y] - BSA and Azide free
    FAK 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP695Y] to FAK - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • IHC-P: human hepatocellular carcinoma

  • 特記事項

    ab271836 is the carrier-free version of ab40794. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab271836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IHC-P
Human
All applications
Mouse
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 119 kDa.

For unpurified use at 1/1000

 
IHC-P
Use at an assay dependent concentration.

The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow.

See IHC antigen retrieval protocols.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 119 kDa.

For unpurified use at 1/1000

 
IHC-P
Use at an assay dependent concentration.

The mouse, rat and cow recommendation is based on the WB results. We do not guarantee IHC-P for mouse, rat and cow.

See IHC antigen retrieval protocols.

ターゲット情報

  • 機能

    Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Microtubule-induced dephosphorylation at Tyr-397 is crucial for the induction of focal adhesion disassembly. Plays a potential role in oncogenic transformations resulting in increased kinase activity.

  • 組織特異性

    Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.

  • 配列類似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
    Contains 1 FERM domain.
    Contains 1 protein kinase domain.

  • ドメイン

    The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL.
    The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.

  • 翻訳後修飾

    Phosphorylated on 6 tyrosine residues upon activation. Microtubule-induced dephosphorylation at Tyr-397 could be catalyzed by PTPN11 and regulated by ZFYVE21. Dephosphorylated by PTPN11 upon EPHA2 activation by its ligand EFNA1.

  • 細胞内局在

    Cell junction > focal adhesion. Cell membrane. Constituent of focal adhesions.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • FADK 1 antibody
    • FADK antibody
    • FAK related non kinase polypeptide antibody
    • FAK1 antibody
    • FAK1_HUMAN antibody
    • Focal adhesion kinase 1 antibody
    • Focal adhesion Kinase antibody
    • Focal adhesion kinase isoform FAK Del33 antibody
    • Focal adhesion kinase related nonkinase antibody
    • FRNK antibody
    • p125FAK antibody
    • pp125FAK antibody
    • PPP1R71 antibody
    • Protein phosphatase 1 regulatory subunit 71 antibody
    • Protein tyrosine kinase 2 antibody
    • Protein-tyrosine kinase 2 antibody
    • Ptk2 antibody
    • PTK2 protein tyrosine kinase 2 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human hepatocellular carcinoma tissue sections labeling FAK with purified ab40794 at 1:250 dilution (2.32 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody.

    Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40794).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using ab40794.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40794).

  • Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)
    Anti-FAK antibody [EP695Y] - BSA and Azide free (ab271836)

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (0)

ab271836 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab271836 は論文での使用が確認できていません。

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