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Anti-FADD 抗体 (ab24533)

Submit a review Q&A (2)References (21)
Western blot - Anti-FADD antibody (ab24533)

    Key features and details

    • Rabbit polyclonal to FADD
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-FADD antibody [EPR4415] (ab108601)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-FADD antibody
      FADD 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to FADD

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide:

      KIDSIEDRYPRNLTERVRESL

      , corresponding to amino acids 125-145 of Human FADD (Peptide available as ab54083.)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called 3039 FADD Antibody. 3039-100 is the same size as the 100 µg size of ab24533.

      FADD has a molecular weight of 23kDa with an apparent molecular weight of 28 kDa on SDS-PAGE.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab24533の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.
    特記事項
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 23 kDa.

    ターゲット情報

    • 機能

      Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.

    • 組織特異性

      Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

    • 配列類似性

      Contains 1 death domain.
      Contains 1 DED (death effector) domain.

    • ドメイン

      Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
    • Information by UniProt

    • 参照データベース

      • 別名

        • FADD antibody
        • FADD protein antibody
        • FADD_HUMAN antibody
        • Fas (TNFRSF6) associated via death domain antibody
        • Fas associated via death domain antibody
        • Fas associating death domain containing protein antibody
        • Fas associating protein antibody
        • Fas associating protein with death domain antibody
        • Fas TNFRSF6 associated via death domain antibody
        • FAS-associated death domain protein antibody
        • FAS-associating death domain-containing protein antibody
        • GIG 3 antibody
        • GIG3 antibody
        • Growth inhibiting gene 3 protein antibody
        • Growth-inhibiting gene 3 protein antibody
        • H sapiens mRNA for mediator of receptor induced toxicity antibody
        • Mediator of receptor induced toxicity antibody
        • MGC8528 antibody
        • MORT 1 antibody
        • MORT1 antibody
        • Protein FADD antibody
        see all

      画像

      • Western blot - Anti-FADD antibody (ab24533)
        Western blot - Anti-FADD antibody (ab24533)
        Lane 1 : Anti-FADD antibody (ab24533) (0.5-4 ug/ml)
        Lane 2 : Anti-FADD antibody (ab24533)

        All lanes : Jurkat cell lysates

        Predicted band size: 23 kDa

      参考文献 (21)

      ab24533 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab24533 は 21 報の論文で使用されています。

      • Cavalcante RS  et al. STAT3/NF-?B signalling disruption in M2 tumour-associated macrophages is a major target of PLGA nanocarriers/PD-L1 antibody immunomodulatory therapy in breast cancer. Br J Pharmacol 178:2284-2304 (2021). PubMed: 33434950
      • Liang X & Ju J Matrine inhibits ovarian cancer cell viability and promotes apoptosis by regulating the ERK/JNK signaling pathway via p38MAPK. Oncol Rep 45:N/A (2021). PubMed: 33786627
      • C de S L Oliveira AL  et al. Effect of Oxaliplatin-Loaded Poly (d,l-Lactide-co-Glycolic Acid) (PLGA) Nanoparticles Combined with Retinoic Acid and Cholesterol on Apoptosis, Drug Resistance, and Metastasis Factors of Colorectal Cancer. Pharmaceutics 12:N/A (2020). PubMed: 32102251
      • Liu W  et al. PI3K/AKT inhibitors aggravate death receptor-mediated hepatocyte apoptosis and liver injury. Toxicol Appl Pharmacol 381:114729 (2019). PubMed: 31445927
      • Sun Y  et al. miR-27a regulates vascular remodeling by targeting endothelial cells' apoptosis and interaction with vascular smooth muscle cells in aortic dissection. Theranostics 9:7961-7975 (2019). PubMed: 31695809
      View all Publications for this product

      レビューと Q&A

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      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Question

      I am trying to source an antibody that works against pig FADD. We have already tried the Stressgen antibody raised against the peptide from the human sequence KIDSIEDRYPPNLTER, and it did not work on pig cell lysates (contrary to the data sheet) but worked on human cell lysates. Upon closer examination, I found that the pig sequence is closer to the mouse peptide in this region and there are quite a number of differece between the pig and human sequences. The abcam antibody ab24533 is raised against a peptide that includes this region, but your website only gives a range for the peptide of aa 100-150 and no precise sequence info. Does your peptide include the region ICDNVGKDWRRLARQLKVSD, which is immediately N terminal to the KIDSIEDRYPPNLTER sequence and a perfect match with the pig (Sus scrofa) sequence? I am basically wanting to know whether or not there is a chance that the abcam antibody will work on pig cells. Also, the dog and pig sequences are almost identical. Thank you

      Read More
      Answer

      Thank you for your patience in this matter. I have received a reply from the originator of ab24533 (FADD antibody). The immunogenic peptide sequence used in raising this product is: KIDSIEDRYPRNLTERVRESL. Please do not hesitate to contact us again if you have further questions.

      Read More

      Question

      What residues does it correspond to on FADD? The product sheet states that it surrounds aa 128 of human FADD. I'm not sure exactly which residues it corresponds to.

      Read More
      Answer

      Thank you for your recent enquiry. I have been informed that the immunogen sequence is within the range of amino acids 100-150 of human FADD, I am unable to provide the specific immunogen sequence as this information is confidential. I hope this information will help you, please do not hesitate to contact us again if you need further assistance,

      Read More

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