製品の概要

  • 製品名
    Anti-Factor XIIIa antibody [AC-1A1]
    Factor XIIIa 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AC-1A1] to Factor XIIIa
  • 由来種
    Mouse
  • アプリケーション
    適用あり: ICC/IF, ELISA, IHC-P, IHC-Fr, WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    BALB/C mice were injected with recombinant human protein corresponding to A-subunit of coagulation Factor XIII.

  • ポジティブ･コントロール
    • Placenta
  • 特記事項

    This antibody recognizes both the dimer and monomer forms.

     

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/25 - 1/50 in an ABC method.
WB Use at an assay dependent concentration. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能
    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
  • 関連疾患
    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.
  • 配列類似性
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • 翻訳後修飾
    The activation peptide is released by thrombin.
  • 細胞内局在
    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt
  • 参照データベース
    • 別名
      • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
      • Coagulation factor XIII A chain antibody
      • Coagulation factor XIII A1 polypeptide antibody
      • Coagulation factor XIII A1 subunit antibody
      • Coagulation factor XIII, A polypeptide antibody
      • Coagulation factor XIIIa antibody
      • F13A antibody
      • F13A_HUMAN antibody
      • F13a1 antibody
      • Factor XIIIA antibody
      • Fibrin stabilizing factor, A subunit antibody
      • Fibrinoligase antibody
      • FSF, A subunit antibody
      • Protein glutamine gamma glutamyltransferase A chain antibody
      • Protein-glutamine gamma-glutamyltransferase A chain antibody
      • TGase antibody
      • Transglutaminase A chain antibody
      • Transglutaminase, plasma antibody
      • Transglutaminase. plasma antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)

      ab1834 - immunohistochemistry

      Formalin fixed paraffin embedded human placenta stained with Factor XIIIa, using ABC and AEC chromogen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      ab1834 staining human placenta by IHC-P.
    • Western blot - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      Western blot - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      Anti-Factor XIIIa antibody [AC-1A1] (ab1834) at 1/250 dilution + Human placenta tissue lysate - total protein (ab29745) at 10 µg

      Secondary
      Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 83 kDa
      Observed band size: 83 kDa
      Additional bands at: 37 kDa, 74 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Factor XIIIa with ab1834.
    • Flow Cytometry - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      Flow Cytometry - Anti-Factor XIIIa antibody [AC-1A1] (ab1834)
      Overlay histogram showing A549 cells stained with ab16956 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16956, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献

    This product has been referenced in:
    • Handrkova H  et al. The activation peptide of coagulation factor XIII is vital for its expression and stability. J Thromb Haemost 13:1449-58 (2015). WB, SDS-PAGE . Read more (PubMed: 26083359) »
    • Mitchell JL  et al. Functional factor XIII-A is exposed on the stimulated platelet surface. Blood 124:3982-90 (2014). WB ; Human . Read more (PubMed: 25331118) »

    See all 7 Publications for this product

    ab1834 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    11 reviews or Q&A

    10
    Votes: Highest First

    I can’t see an attachment with the questionnaire but I have attached my protocol.

    The issue that I am having is the AC-1A1 antibody (cat no. Ab-1834) does not detect the factor XIII-A subunit in normal platelet free plasma. The antibody ...

    Read More

    Thank you for your phone call and email.

    I apologize as I forget to attach the questionnaire. I have looked into the details and would like to make following suggestions;

    ab1834 was able to detect band in Fibrogammin samples so I assu...

    Read More

    Antibody fials to detect Factor 13 in WB.

    Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As we discussed on the ph...

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    Please see customer`s request below:



    We have recently purchased ab1834 Anti-Factor XIIIa. We are wondering if you could provide us with some general guidelines for use with human paraffin immunohistochemistry. Specifically we wou...

    Read More

    Thank you for your email.

    This antibody was used in the following publication:

    Ulici V et al. Regulation of gene expression by PI3K in mouse growth plate chondrocytes. PLoS One 5:e8866 (2010). WB, IHC-P; Mouse. (PubMed: 20111593) Read More

    Dear Kate

    We will be using the primary and secondary antibodies for staining formalin fixed, paraffin embedded tissue sections.
    I asked Peter for a discount on the primary antibodies and shipping charge and he also offered the secondary...

    Read More

    Thank you for your reply.

    As ab125687 PAI1 antibody [1D5], ab1834 Factor XIIIa antibody [AC-1A1] and ab54488 CD41/Integrin alpha 2b antibody [283.16B7] are all mouse IgG1, Ican suggest to consider the following anti mouse IgG1 secondaries av...

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    Details of this antibody by western blotting - Does it require NON-REDUCING conditions?

    Thank you for your enquiry. Further to our liveperson discussion yesterday morning I have performed some literature searching and in the following publication factor XIIIA was studied using BOTH reducing and non-reducing conditions. Whilst it is ch...

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    I have a customer's enquiry regarding ab1834. She wants to used this antibody for IHC-Paraffin, she would like to know what is the best antigen retrieval method, eg enzymatic retrieval. I told her about the method using chymotrypsin, however she was w...

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    Thank you for your enquiry regarding ab1834 and your interest in our products. This particular antibody to Factor XIIIa does not require any antigen retrieval for paraffin sections. Proteinase K is more suitable for in situ hybridization and not for...

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    Please, could you give me the used dilution, in Western Blot, for your antibody ab1834 ? and some references in the litterature concerning this antibody ?

    Thank you for your email. I would suggested diluting ab1834 to 1:1000 and optimizing based upon your results. We're unfortunately not aware of any publications which feature the use of this antibody. If you have any additional questions, please do ...

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    I am carrying out research on fibrous lesions of the oral mucosa and am interested in using ab1834 (anti Factor XIIIa) on formalin fixed paraffin-enbedded tissue. Since these are very fibrous lesions, I have in the past experienced problems with loss o...

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    Thank you for your enquiry. You do not need any pretreatment when staining with this particular antibody. It just works fine. However, if your tissue is over fixed, you need enzymatic treatment.

    Was the recombinant protein used to make the immunogen affinity purified?

    Recombinant protein was purified using HPLC.

    I will like to know the IgG protein concentration of ab1834?Have you measured it?

    The concentration is 100 ug/ml.

    1-10 of 11 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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