Anti-Factor XIIIa 抗体 (ab4057)

Reviews (2)Q&A (4)

Key features and details

  • Rabbit polyclonal to Factor XIIIa
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Factor XIIIa antibody
    Factor XIIIa 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Factor XIIIa

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant protein corresponding to subunit-A of coagulation Factor XIIIa.

  • ポジティブ･コントロール

    • Placenta.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4057 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Incubate for 30 minuts at room temperature (ABC method).
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.

  • 関連疾患

    Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women.

  • 配列類似性

    Belongs to the transglutaminase superfamily. Transglutaminase family.

  • 翻訳後修飾

    The activation peptide is released by thrombin.

  • 細胞内局在

    Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma.
  • Information by UniProt

  • 参照データベース

    • 別名

      • bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
      • Coagulation factor XIII A chain antibody
      • Coagulation factor XIII A1 polypeptide antibody
      • Coagulation factor XIII A1 subunit antibody
      • Coagulation factor XIII, A polypeptide antibody
      • Coagulation factor XIIIa antibody
      • F13A antibody
      • F13A_HUMAN antibody
      • F13a1 antibody
      • Factor XIIIA antibody
      • Fibrin stabilizing factor, A subunit antibody
      • Fibrinoligase antibody
      • FSF, A subunit antibody
      • Protein glutamine gamma glutamyltransferase A chain antibody
      • Protein-glutamine gamma-glutamyltransferase A chain antibody
      • TGase antibody
      • Transglutaminase A chain antibody
      • Transglutaminase, plasma antibody
      • Transglutaminase. plasma antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody (ab4057)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody (ab4057)This image is courtesy of an anonymous Abreview

      ab4057 staining formalin fixed paraffin-embedded human bowel tumour tissue sections.  The sample was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 10% srum for 1 hour at 24°C, prior to incubation with the primary antibody (diluted 1/100 in milk) for 12 hours at 4°C.  A biotinylated goat anti-rabbit antibody was used as the secondary.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody (ab4057)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor XIIIa antibody (ab4057)
      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, staining Factor XIIIa with ab4057.

    参考文献 (0)

    ab4057 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4057 は論文での使用が確認できていません。

    レビューと Q&A

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    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Factor XIIIa antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (spinal cord)
    Specification
    spinal cord
    Fixative
    Acetone
    Permeabilization
    Yes - 0,05% Triton
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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    Abcam user community

    Verified customer

    投稿 Apr 24 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Factor XIIIa antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (bowel tumor)
    Specification
    bowel tumor
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrat puffer ph 6
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 08 2008

    Question

    Thank you for your prompt reply. If you wouldn't mind, I have some more questions. I know that Factor VIII related antigen also stains endothelial cells. Can these 2 things be used interchangablely? It seems strange that we would be instructed to test Factor XIIIa on placenta. Is there something special about placental vessels?

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    Answer

    Thank you for your enquiry. Both proteins are involved in the coagulation cascade. Whether you can use the antibodies interchangeably depends on the experiments you are doing. Unfortunately we aren't experts in the field, so we couldn't tell you which one would be better to use in your particular situation. I am not sure why placenta is suggested as the positive control. It is possible that the protein shows higher expression in placental blood vessels than vessels in other tissues. If you would like me to enquire further about this matter, I would be happy to. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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    Question

    The positive control listed for this antibody is placenta. Which placental cells should I use? In addition, the localization is listed as cytoplasmic and nuclear. Which localization gives more sensitive staining?

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    Answer

    Thank you for your enquiry. Factor XIIIa is a coagulation protein present in the plasma. Staining is cytoplasmic. It should stain the endothelial cells of the blood vessels. It appears that the datasheet states that the cellular localization is cytoplasmic and secreted, not cytoplasmic and nuclear. I completely missed that, and I apologize. I hope this information helps. Please do not hesitate to contact us if you need anything further.

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    Question

    No, i didn't try placenta due to the absence of this tissue in our laboratory. Placenta is also recommended as a positive control in the protocol provided by Novocastra. In addition to it, skin can be used as a positive control too since dermal dendritic cells are immunoreactive to Factor XIIIa AB (ref. Novocastra catalog) and upregulation of this cell type is well described in different inflammatory conditions. I hope this information is helpful

    Read More
    Answer

    Thank you for your response. This antibody has been tested for IHC using human placenta so it would be very important to run an experiment with tested positive control alongside with the samples. As you have mentioned in your e-mail, dendritic cells also express Factor XIIIa. However, the expressed levels of Factor XIIIa may be different from that in the placenta which usually shows very high levels of this protein. We have looked through our order record and actually the batch number you received was 77421. We have sold many vials form this batch without any problem. It may well be that the vial you received has become inactivated during shipping and/or storage. We can offer you a new vial free of charge. Please do let us know if you are interested in, and then we can place a new order for you as soon as possible.

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    Question

    I would like to use the AB in mouse. How likely is a crossreactivity with mouse FXIII?

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    Answer

    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with mouse has not yet been tested for use with ab4057. Should you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher. Please let me know if you require any further assistance.

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