Anti-Factor VII 抗体 (ab97614)
Key features and details
- Rabbit polyclonal to Factor VII
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Factor VII antibody
Factor VII 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Factor VII -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Monkey
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免疫原
Recombinant protein fragment corresponding to a region within amino acids 209 and 444 of Human Factor VII.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97614の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 52 kDa.
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| IHC-P |
1/100 - 1/500.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 52 kDa. |
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IHC-P
1/100 - 1/500. |
ターゲット情報
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機能
Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium. -
組織特異性
Plasma. -
関連疾患
Defects in F7 are the cause of factor VII deficiency (FA7D) [MIM:227500]. FA7D is a rare hereditary hemorrhagic disease. The clinical picture can be very severe, with the early occurrence of intracerebral hemorrhages or hemarthroses, or, in contrast, moderate with cutaneous-mucosal hemorrhages (epistaxis, menorrhagia) or hemorrhages provoked by a surgical intervention. Numerous subjects are completely asymptomatic despite a very low F7 level. -
配列類似性
Belongs to the peptidase S1 family.
Contains 2 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 1 peptidase S1 domain. -
翻訳後修飾
The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 2155 Human
- Entrez Gene: 14068 Mouse
- Omim: 613878 Human
- SwissProt: P08709 Human
- SwissProt: P70375 Mouse
- Unigene: 36989 Human
- Unigene: 4827 Mouse
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別名
- coagulation factor VII (serum prothrombin conversion accelerator) antibody
- Coagulation factor VII antibody
- Eptacog alfa antibody
see all
画像
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Western blot - Anti-Factor VII antibody (ab97614)Anti-Factor VII antibody (ab97614) at 1/1000 dilution + NIH 3T3 whole cell lysate at 30 µg
Predicted band size: 52 kDa
10% SDS PAGE -
Western blot - Anti-Factor VII antibody (ab97614)Anti-Factor VII antibody (ab97614) at 1/500 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 52 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor VII antibody (ab97614)Immunohistochemistry analysis of paraffin-embedded N human (gastric carcinoma) tissue, using ab97614 at 1/100 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab97614 は 8 報の論文で使用されています。
- Wypasek E et al. Effects of rivaroxaban and dabigatran on local expression of coagulation and inflammatory factors within human aortic stenotic valves. Vascul Pharmacol 130:106679 (2020). PubMed: 32387621
- Mao XB et al. miR-204-5p promotes diabetic retinopathy development via downregulation of microtubule-associated protein 1 light chain 3. Exp Ther Med 17:2945-2952 (2019). PubMed: 30936964
- Mashayekhi A et al. Functional and Molecular Characterization of C91S Mutation in the Second Epidermal Growth Factor-Like Domain of Factor VII. Iran J Biotechnol 16:e1813 (2018). PubMed: 30555841
- Mashayekhi A et al. In vitro expression of mutant factor VII proteins and characterization of their clinical significance. Mol Med Rep 17:2738-2742 (2018). PubMed: 29207137
- Zhang S et al. Synergistic Inhibitory Effect of Traditional Chinese Medicine Astragaloside IV and Curcumin on Tumor Growth and Angiogenesis in an Orthotopic Nude-Mouse Model of Human Hepatocellular Carcinoma. Anticancer Res 37:465-473 (2017). PubMed: 28179291
- Huang KT et al. Factor VII-Induced MicroRNA-135a Inhibits Autophagy and Is Associated with Poor Prognosis in Hepatocellular Carcinoma. Mol Ther Nucleic Acids 9:274-283 (2017). PubMed: 29246306
- Nagahashi K et al. Successful synthesis of active human coagulation factor VII by co-expression of mammalian gamma-glutamyl carboxylase and modification of vit.K cycle in Drosophila Schneider S2 cells. Cytotechnology 69:317-327 (2017). PubMed: 28070807
- Chen KD et al. Interconnections between autophagy and the coagulation cascade in hepatocellular carcinoma. Cell Death Dis 5:e1244 (2014). WB ; Human . PubMed: 24853422