Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (adipose-derived stem cells for adipogenesis)
Permeabilization
Yes - 0.1% Triton X-100 made in PBS solution for 15 min
Specification
adipose-derived stem cells for adipogenesis
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat anti-rabbit econdary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Permeabilization of cells with 0.1% Triton X-100 made in PBS solution for 15 min.
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投稿 Apr 14 2015