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  1. Link

    fabp4-antibody-ab13979.pdf

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Cardiovascular Lipids / Lipoproteins Fatty Acids Binding proteins
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Anti-FABP4 抗体 (ab13979)

  • Datasheet
  • SDS
Reviews (2)Q&A (6)References (32)

Product price, shipping and contact information

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)

Key features and details

  • Rabbit polyclonal to FABP4
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-FABP4 antibody [EPR3579] (ab92501)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-FABP4 antibody
    FABP4 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to FABP4
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-Pmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Recombinant full length protein (Human).

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Constituents: 0.134% PBS, 0.58% Sodium chloride
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Binding proteins
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Stem Cells
    • Mesenchymal Stem Cells
    • Adipogenesis
    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Types of disease
    • Cancer
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human FABP4 protein (ab63283)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13979の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
1/200.
特記事項
IHC-P
1/200.

ターゲット情報

  • 機能

    Lipid transport protein in adipocytes. Binds both long chain fatty acids and retinoic acid. Delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus.
  • 配列類似性

    Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
  • ドメイン

    Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior.
  • 細胞内局在

    Cytoplasm. Nucleus. Depending on the nature of the ligand, a conformation change exposes a nuclear localization motif and the protein is transported into the nucleus. Subject to constitutive nuclear export.
  • Target information above from: UniProt accession P15090 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2167 Human
    • Omim: 600434 Human
    • SwissProt: P15090 Human
    • Unigene: 391561 Human
    • 別名

      • 3T3-L1 lipid-binding protein antibody
      • 422/aP2 antibody
      • A-FABP antibody
      • adipocyte antibody
      • Adipocyte lipid binding protein antibody
      • Adipocyte lipid-binding protein antibody
      • Adipocyte protein AP2 antibody
      • Adipocyte-type fatty acid-binding protein antibody
      • AFABP antibody
      • ALBP antibody
      • ALBP/Ap2 antibody
      • aP2 antibody
      • Epididymis secretory protein Li 104 antibody
      • FABP antibody
      • FABP4 antibody
      • FABP4_HUMAN antibody
      • Fatty acid binding protein 4 adipocyte antibody
      • Fatty acid binding protein 4 antibody
      • Fatty acid binding protein adipocyte antibody
      • Fatty acid-binding protein 4 antibody
      • Fatty acid-binding protein antibody
      • HEL S 104 antibody
      • Lbpl antibody
      • Myelin P2 protein homolog antibody
      • P15 antibody
      • P2 adipocyte protein antibody
      • Protein 422 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)

      Immunohistochemical analysis of FFPE human adipose tissue, labeling FABP4 with ab13979 (2µg/ml). DAB staining.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)Image from Holm S et al., PLoS One. 2011;6(12):e28785. Epub 2011 Dec 9. Fig 3.; doi:10.1371/journal.pone.0028785; December 9, 2011, PLoS ONE 6(12): e28785.
      Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.

      Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. Staining was detected using DAB.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)This image is courtesy of an anonymous Abreview.

      Immunohistochemical analysis of FFPE mouse pancreatic and peri-pancreatic fatty tissue, labeling FABP4 with ab13979. Tissue was fixed in 10% neutral buffered formalin, heat mediated antigen retrieval was performed using citrate buffer, and blocking was performed with 10% goat serum and 1% BSA inj TBST for 1 hour at 25°C. 

      Staining with ab13979 (diluted 1/500) was performed for 12 hours at 4°C. A goat anti-rabbit HRP-conjugated secondary was used, diluted 1/200.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody (ab13979)Image from Holm S et al., PLoS One. 2011;6(12):e28785. Epub 2011 Dec 9. Fig 3.; doi:10.1371/journal.pone.0028785; December 9, 2011, PLoS ONE 6(12): e28785.
      Immunohistochemical analysis of Human carotid atherosclerotic plaques, staining FABP4 with ab13979.

      Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) and blocked with 0.5% BSA. Sections were incubated with primary antibody for 1 hour at room temperature. An AlexaFluor®488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (32)

    ab13979 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab13979 は 32 報の論文で使用されています。

    • Miao L  et al. FABP4 deactivates NF-?B-IL1a pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression. Clin Transl Med 11:e395 (2021). PubMed: 33931964
    • Liu X  et al. Type I collagen inhibits adipogenic differentiation via YAP activation in vitro. J Cell Physiol 235:1821-1837 (2020). PubMed: 31432516
    • Cen S  et al. TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2. EBioMedicine 54:102722 (2020). PubMed: 32268273
    • Chou YT  et al. Wwox Deficiency Causes Downregulation of Prosurvival ERK Signaling and Abnormal Homeostatic Responses in Mouse Skin. Front Cell Dev Biol 8:558432 (2020). PubMed: 33195192
    • Takahashi N  et al. ROCK Inhibition Induces Terminal Adipocyte Differentiation and Suppresses Tumorigenesis in Chemoresistant Osteosarcoma Cells. Cancer Res 79:3088-3099 (2019). PubMed: 30992323
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-FABP4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Heart)
    Permeabilization
    Yes - Tween-20
    Specification
    Heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 10 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-FABP4 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas and peri-pancreatic fatty tissue)
    Specification
    pancreas and peri-pancreatic fatty tissue
    Fixative
    10% NBF
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate
    Permeabilization
    No
    Blocking step
    10% goat serum/1%BSA in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 11 2010

    Question


    I ordered anti-FABP4 (ab13979) from abcam and now I am wondering which
    secondary antibody should I use for detection? I want to use an alexa
    fluor (e.g 488), but I don't know which species? In the images at you
    webpage for this antibody somebody had used alexa fluor conjugated
    goat anti-rabbit IgG, is it so that anti-rabbit secondary antibodies
    function with this FABP4 antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Oct 23 2012

    Answer

    Thank you for contacting us.

    The antibody you have ordered is a rabbit polyclonal to FABP4 therefore, you will need to use an anti-rabbit secondary antibody so that it can recognise and bind to your primary antibody.

    Unfortunately we do not currently stock alexa fluorophores however I can recommend you use Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Chromeo™ 488) (ab60314) as Chromeo™ 488 is spectraly similar to Alexa® 488.

    Alternatively, you could also try our range of DyLight secondary antibodies:


    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-ab96883.html
    https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-ab96883.html

    https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-ab96891.html
    https://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-ab96891.html

    https://www.abcam.com/Sheep-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-ab96923.html
    https://www.abcam.com/Sheep-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-ab96923.html





    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 23 2012

    Question

    Replacement vial from same lot gives multiple bands in western blots.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 19 2012

    Answer

    I have issued a free of charge replacement with the order number **** for the rabbit monoclonal anti-FABP4, ab92501.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Jun 19 2012

    Question

    High background staining in negative control kidney tissue. Requests refund.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 14 2012

    Answer

    Thank you for your call today and for letting us know about the trouble with this antibody.

    Your credit note ID is ***.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone at 888-772-2226. Please refer to the credit note ID in any correspondence with our accounting department.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require any further assistance.

    Read More

    Abcam Scientific Support

    Answered on Jun 14 2012

    Question

    We have been using this antibody for several years and the most recent lot is not working in WB. Can we please receive a replacement?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 01 2012

    Answer

    Thank you for contacting Abcam regarding ab13979.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Jun 01 2012

    Question

    Phone call reporting non-specific staining using anti-FABP4 antibody (ab13979).

    Read More

    Abcam community

    Verified customer

    Asked on May 21 2012

    Answer

    Thank you for calling us and for alerting us to the problem you are experiencing with our product. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

    As we discussed on the phone, I amattaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. OnceI have receive the completed questionnaire,I will look at the protocol and see if there are any suggestionsI can make that may improve the results. This information will also allow us to investigate this case internally and initiate any additional testing where necessary.

    If the product was purchased in the last six months andwas being used according to our Abpromise, we would be happy to replace or refund the antibody.

    As discussed over the phone, I am looking into exactly what protocol has been used with this antibody previously and if we have any other suitable alternatives for you.

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on May 21 2012

    Question

    Hi, As to FABP4 antibody (ab13979), I'd like to know that whetehr this antibody works in zebrafish. May I know what the sequence of the peptide you used to immunize the rabbit?

    Read More

    Abcam community

    Verified customer

    Asked on May 18 2011

    Answer

    Thank you for your enquiry. The immunogen used to make this antibody was not a peptide but the full length protein. SwissProt link (http://www.uniprot.org/uniprot/P15090) I have taken the liberty BLASTing the sequence with zebrafish to check for homology (see attached PDF file). Unfortunately, there does not seem to be any hits for FABP4 protein and I can't be sure if this product could or could not work for zebrafish. Please bear in mind that this product has not been tested to work in zebrafish, therefore we are unable to guarantee that you will be able to obtain good results. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on May 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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