Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse spleen)
Permeabilization
No
Specification
Mouse spleen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: rt°C
Fixative
Acetone
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Does not work on OCT frozen mouse spleen tissue.
Abcam response
Thank you so much for testing this antibody in IHC-Fr. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this application.
We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Feb 28 2018