Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton-X 100
Specification
kidney
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% DS/ 0.1% Triton-X 100
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Donkey anti Rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Additional data
Additional Notes
4% PFA fixative
Abcam response
Thank you so much for testing this antibody in rat. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this species.
We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 28 2017