製品の概要

  • 製品名

    Anti-F4/80 antibody [SP115]
    F4/80 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [SP115] to F4/80
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, Flow Cyt, IHC-FrFlmore details
  • 種交差性

    交差種: Mouse
  • 免疫原

    Synthetic peptide within Mouse F4/80 aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q61549

  • ポジティブ・コントロール

    • IHC-P: Mouse colon, liver and lung tissue; M1 and M2 macrophages from mice colon tissue. FC: Raw264.7 cells
  • 特記事項

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111101 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100.

For antigen retrieval: Boil tissue section in EDTA buffer for 10 min followed by cooling at RT for 20 min.

Flow Cyt 1/20.
IHC-FrFl Use at an assay dependent concentration. PubMed: 24647450

ターゲット情報

  • 機能

    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 組織特異性

    Expression is restricted to eosinophils.
  • 配列類似性

    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 細胞内局在

    Cell membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling F4/80 with ab111101 at 1/250 dilution (0.48 µg/ml). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on macrophages in the mouse liver.

  • Flow cytometry analysis of Raw264.7 (mouse abelson murine leukemia virus-induced tumor) labeling F4/80 with purified ab111101 at 1/20 dilution (6.05 µg/ml) (red). Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Immunohistochemistry analysis of mouse skin labeling F4/80 with ab111101 at 1/200 dilution by an overnight incubation at room temperature after blocking endogenous peroxidase and nonspecific binding.  After washing in PBS, the sections were exposed to biotinylated goat anti-rabbit IgG (1/200 dilution, ab150077) for 1 h at room temperature. Results show significant difference between tiger17 treatment and control.

  • Representative immunostaining of F4/80-positive macrophages in the distal colon from healthy and colitic mice treated with and without enoxaparin.

    For immunohistochemical staining, antigen retrieval was performed by incubating the sections for 10 minutes at 97°C in 1 mM EDTA buffer, pH 8 or 10 mM citrate buffer, pH 6. Activity of endogenous peroxidase was blocked by incubating sections with 3% v/v hydrogen for 20 minutes. Sections were then washed with 0.05 M Tris-buffered saline containing 0.5% v/v Tween 20 (TBST), pH 7.6. Subsequently, sections were incubated with serum-free protein block for 10 minutes. Colon sections were then incubated with primary antibody ab111101 at 1/100 dilution overnight at 4°C or room temperature for 1 hour. Sections were then washed 3 x 5 minutes and allowed to react with secondary antibody: anti-rabbit immunoglobulin C conjugated to horseradish peroxidase (HRP) (ab7090) at 1/300 dilution at room temperature for 1 hour.

    Scale bar = 100 μm for 400 x magnification. Control, C; untreated colitis, DSS; oral enoxaparin, OE; intraperitoneal injection of enoxaparin, IPE.

  • Representative images of (A) M1 macrophages (F4/80+ and iNOS+) and (B) M2 macrophages (F4/80+ and CD206+) using colon tissue from n = 3–5 mice. F4/80 positive cells were visualized using Alexa Fluor 594-conjugated goat anti-rat IgG (red). Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, blue).

    Scale bar = 50 μm for 400 × magnification. Control, C; untreated colitis, DSS; colitis with oral enoxaparin, DSS+OE.

    For immunofluorescence staining, sections were dewaxed and rehydrated before antigen retrieval using 10 mM citrate buffer, pH 6 for 15 minutes at 97°C. Sections were incubated with serum-free protein block and permeabilized with 0.4% v/v Triton-X at room temperature for 30 minutes. Sections were incubated with primary antibodies anti-F4/80 (ab16911) at 1/25 dilution overnight at 4°C or at room temperature for 1 hour. Sections were washed with TBST 3 × 10 minutes and incubated with species-specific secondary antibodies: anti-rat IgG H&L AlexaFluor 594 (ab150160, Abcam, 1:1000) and anti-rabbit IgG H&L AlexaFluor 488 (A11070, Thermo Fisher Scientific, Melbourne, Australia, 1:1000) at room temperature for 2 hours. Sections were rinsed with TBST 3 × 10 minutes, followed by a quick wash with distilled water before mounting using Glycerol Mounting Medium (Abcam) that contained 4’,6-diamidino-2-phenylindole (DAPI) and 1,4-diazobicyclo-2,2,2-octane (DABCO). Labelled tissues were visualized using a Leica DM LB2 microscope. Fluorescence images (400 × magnification) were captured using NIS-Elements 4.13 (Nikon) software. 

    For full image see PMID: 26218284.

  • ab111101 at 1/100 dilution staining F4/80 in Formalin-fixed, paraffin-embedded Mouse liver tissue.

  • Immunohistochemistry analysis of Formalin fixed paraffin-embedded mouse lung tissue sections labeling F4/80 with ab111101 at 1/200 for 16 hours at 4°C. Biotin conjugated Goat anti-rabbit polyclonal antibody at 1/500 was used as the secondary. Antigen retrieval was heat mediated using citrate buffer pH 6.0. 

    See Abreview

  • Immunohistochemical analysis staining for macrophages in (A) mouse uterus and (B) mouse spleen using ab111101 at a dilution of 1:200. HRP Anti-Rabbit IgG (Peroxidase) Polymer D antibody was used as a secondary. 

    See Abreview

プロトコール

参考文献

This product has been referenced in:

  • Ghanem LY  et al. Liver Macrophage Depletion Ameliorates The Effect of Mesenchymal Stem Cell Transplantation in a Murine Model of Injured Liver. Sci Rep 9:35 (2019). Read more (PubMed: 30631109) »
  • Saleh Y  et al. Exposure to Atmospheric Ultrafine Particles Induces Severe Lung Inflammatory Response and Tissue Remodeling in Mice. Int J Environ Res Public Health 16:N/A (2019). Read more (PubMed: 30987320) »
See all 36 Publications for this product

レビューと Q&A

1-10 of 27 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
Yes - Tween-20
Specification
Uterus
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Paraformaldehyde

Reshef Tal

Verified customer

投稿 Sep 05 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Bone)
Permeabilization
Yes - 0.05% Triton-100X
Specification
Bone
Blocking step
Vetor Blocker as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde

Dr. Hanson Lee

Verified customer

投稿 Aug 19 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (tumor tissue)
Permeabilization
Yes - Tx 100 0.1%
Specification
tumor tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
Fixative
Methanol

Abcam user community

Verified customer

投稿 Aug 08 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Liver)
Permeabilization
No
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Jun 13 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse spleen)
Permeabilization
No
Specification
Mouse spleen
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: rt°C
Fixative
Acetone

Abcam user community

Verified customer

投稿 Feb 28 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spleen)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: ProK
Permeabilization
No
Specification
Spleen
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Feb 19 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (kidney)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton-X 100
Specification
kidney
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Nov 28 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (uterus and spleen (multi-block))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako high pH buffer
Permeabilization
No
Specification
uterus and spleen (multi-block)
Blocking step
Vector casein solution as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde

Dr. Henry King

Verified customer

投稿 Aug 07 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

投稿 Jul 11 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (rat blood vessels)
Antigen retrieval step
None
Permeabilization
No
Specification
rat blood vessels
Blocking step
Dako blocking solution as blocking agent for 45 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 Mar 01 2017

1-10 of 27 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

登録