Anti-F4/80 抗体 [CI:A3-1] (Phycoerythrin) (ab105156)


  • 製品名
    Anti-F4/80 antibody [CI:A3-1] (Phycoerythrin)
    F4/80 一次抗体 製品一覧
  • 製品の詳細
    Rat monoclonal [CI:A3-1] to F4/80 (Phycoerythrin)
  • 由来種
  • 標識
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • 特異性
    ab105156 recognises the murine F4/80 antigen.
  • アプリケーション
    適用あり: Flow Cyt, IHC-Fr, ICC/IFmore details
  • 種交差性
    交差種: Mouse
  • 免疫原

    Thioglycollate stimulated peritoneal cavity macrophages from C57/BL mice.

  • ポジティブ・コントロール
    • Mouse J774 cells
  • 特記事項

    Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.


  • 製品の状態
  • 保存方法
    Shipped at 4°C. Store at +4°C.
  • バッファー
    pH: 7.40
    Preservative: 0.09% Sodium azide
    Constituents: 1% BSA, PBS, 5% Sucrose
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 一次抗体 備考
    Clone CI:A3-1 has been reported to modulate cytokine levels released in response to Listeria monocytogenes.
  • ポリ/モノ
  • クローン名
  • ミエローマ
  • アイソタイプ
  • 研究分野


Our Abpromise guarantee covers the use of ab105156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 10µl for 106 cells.

in 100 µl.

ab136585 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.


  • 機能
    Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
  • 組織特異性
    Expression is restricted to eosinophils.
  • 配列類似性
    Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
    Contains 6 EGF-like domains.
    Contains 1 GPS domain.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADGRE1 antibody
    • Adhesion G protein coupled receptor E1 antibody
    • Adhesion G protein-coupled receptor E1 antibody
    • AGRE1_HUMAN antibody
    • Cell surface glycoprotein EMR1 antibody
    • Cell surface glycoprotein F4/80 antibody
    • DD7A5 7 antibody
    • Egf like module containing mucin like hormone receptor like 1 antibody
    • Egf like module containing mucin like hormone receptor like sequence 1 antibody
    • EGF like module receptor 1 antibody
    • EGF TM7 antibody
    • EGF-like module receptor 1 antibody
    • EGF-like module-containing mucin-like hormone receptor-like 1 antibody
    • EGFTM7 antibody
    • EMR 1 antibody
    • EMR1 hormone receptor antibody
    • Gpf480 antibody
    • Ly71 antibody
    • Lymphocyte antigen 71 antibody
    • TM7LN3 antibody
    see all


  • Flow cytometry staining of mouse J774 cells with ab105156.


This product has been referenced in:
  • Li H  et al. Fibroblast growth factor 21 increases insulin sensitivity through specific expansion of subcutaneous fat. Nat Commun 9:272 (2018). Read more (PubMed: 29348470) »
  • Neng L  et al. Isolation and culture of endothelial cells, pericytes and perivascular resident macrophage-like melanocytes from the young mouse ear. Nat Protoc 8:709-20 (2013). Read more (PubMed: 23493068) »

See all 2 Publications for this product

レビューと Q&A

Vielen Dank für Ihre Anfrage.

Leider ist es nicht möglich die Konzentration von ab105156 zu bestimmen.

Bei ab105156 handelt es sich einen konjugierten Antikörper.

Die Konzentration von Antikörpern wird ...

Read More

Thank you for your inquiry.

I can confirm that the volume of ab105156 is 500ul per vial. The concentration cannot be determined since this a PE-conjugated antibody. An estimatewould be ˜ 0.1mg/ml.

I suggest to use two concentrations...

Read More

Thank you for your query. I can confirm that the unconjugated clone of ab105156 was typically used at around 10ug/ml for frozen sections. However, I would still recommend to titrate the antibody in order to determine the optimal concentration f...

Read More

Thank you for your interest. Just to confirm ab105156 is a primary antibody which is conjugated to PE. This means that it is not necessary to apply a compatible secondary antibody for detection. For further details (tested applications,...

Read More

Thank you for your inquiry. We have a number of antibodies in our catalog that might be of interest to you. I would like to direct you to the search facility on our website ( Please enter the name of the protein into the search box a...

Read More