Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow-derived macrophages)
Permeabilization
Yes - Triton X-100
Specification
Bone marrow-derived macrophages
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Rat IgG Secondary Antibody, Alexa F
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 14 2017