Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Brain (50um))
Antigen retrieval step
None
Permeabilization
Yes - 3 x 5mn PBS-T
Specification
Brain (50um)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Alexa Fluor® 594 AffiniPure Goat Anti-Rat IgG (H+L
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Picture represents part of the Thalamus - Objective Magnification = 40x - Apotome has been used. Red channel = F4/80 Green channel = Iba1
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jun 04 2019