Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Jaculus jaculus Tissue sections (spleen)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: proteaseK
Permeabilization
No
Specification
spleen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Alexa anti rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 594
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mai Tran
Verified customer
投稿 Jul 12 2016