Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Mouse Cell (peritoneal macrophages, RAW 264.7)
Specification
peritoneal macrophages, RAW 264.7
Permeabilization
Yes - 0.1% Saponin
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: 3% BSA, 0.1% Saponin in PBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 555 Goat Anti-Rat IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/1000
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投稿 Aug 18 2014