Anti-F4/80 抗体 (ab100790)
Key features and details
- Rabbit polyclonal to F4/80
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-F4/80 antibody
F4/80 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to F4/80 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Mouse
交差が予測される動物種: Rat非交差種: Human -
免疫原
Synthetic peptide within Mouse F4/80 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q61549 -
ポジティブ・コントロール
- IHC-P: Mouse Liver tissue
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab100790の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P | (4) |
1/100.
Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. This product may benefit from the use of freshly cut tissue. |
特記事項 |
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IHC-P
1/100. Boil tissue section in 1mM EDTA, pH 8.0 for 10 min followed by cooling at RT for 20 min. This product may benefit from the use of freshly cut tissue. |
ターゲット情報
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機能
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development. -
組織特異性
Expression is restricted to eosinophils. -
配列類似性
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
Contains 6 EGF-like domains.
Contains 1 GPS domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 13733 Mouse
- Entrez Gene: 316137 Rat
- SwissProt: Q61549 Mouse
- SwissProt: Q5Y4N8 Rat
- Unigene: 2254 Mouse
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別名
- ADGRE1 antibody
- Adhesion G protein coupled receptor E1 antibody
- Adhesion G protein-coupled receptor E1 antibody
see all
画像
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IHC image of F4/80 staining in a section of formalin-fixed paraffin-embedded normal mouse liver performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA buffer (pH9, epitope retrieval solution 2) for 20mins. The section was then incubated with ab100790, 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (84)
ab100790 は 84 報の論文で使用されています。
- Du M et al. The role of long noncoding RNA Nron in atherosclerosis development and plaque stability. iScience 25:103978 (2022). PubMed: 35295812
- Chen C et al. Engineering an enthesis-like graft for rotator cuff repair: An approach to fabricate highly biomimetic scaffold capable of zone-specifically releasing stem cell differentiation inducers. Bioact Mater 16:451-471 (2022). PubMed: 35386315
- Araujo N et al. Tumor Suppressor Par-4 Regulates Complement Factor C3 and Obesity. Front Oncol 12:860446 (2022). PubMed: 35425699
- Srivastava RK et al. Role of hair follicles in the pathogenesis of arsenical-induced cutaneous damage. Ann N Y Acad Sci 1515:168-183 (2022). PubMed: 35678766
- Zhu Q et al. N-acetylcysteine attenuates atherosclerosis progression in aging LDL receptor deficient mice with preserved M2 macrophages and increased CD146. Atherosclerosis 357:41-50 (2022). PubMed: 36041358