Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton-x
Specification
spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/ 0,3% Triton-x / 1%BSA
Dilution
1/100
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: 111-065-144 goat IgG anti Rabbit IgG (H+L) Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Abcam response
Thank you for participating in our AbTrial program and your valuable feedback about the use of ab126629 in mouse. Based on our internal testing and the researchers feedback, ab126629 may not be suitable for mouse samples and we recommend ab176573 for this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 01 2017