Anti-Exonuclease 1 抗体 (ab95012)
Key features and details
- Rabbit polyclonal to Exonuclease 1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Exonuclease 1 antibody
Exonuclease 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Exonuclease 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human Exonuclease 1 aa 700-800. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_006018.4
-
ポジティブ・コントロール
- HeLa and 293T whole cell lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
ab95012 was affinity purified using an epitope specific to Exonuclease 1 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95012の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 94 kDa.
|
|
IP |
Use at 5-15 µg/mg of lysate.
|
特記事項 |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 94 kDa. |
IP
Use at 5-15 µg/mg of lysate. |
ターゲット情報
-
機能
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. -
組織特異性
Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach. -
配列類似性
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily. -
発生段階
Highly expressed in fetal liver and at lower levels in fetal brain, heart, kidney, spleen and thymus. -
翻訳後修飾
Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein. -
細胞内局在
Nucleus. Colocalizes with PCNA to discrete nuclear foci in S-phase. - Information by UniProt
-
参照データベース
- Entrez Gene: 457856 Chimpanzee
- Entrez Gene: 9156 Human
- Omim: 606063 Human
- SwissProt: Q9UQ84 Human
- Unigene: 498248 Human
-
別名
- exo1 antibody
- EXO1_HUMAN antibody
- ExoI antibody
see all
画像
-
All lanes : Anti-Exonuclease 1 antibody (ab95012) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 94 kDa
Exposure time: 30 seconds -
ab95012 at 1 µg/ml detecting Exonuclease 1 in HeLa whole cell lysate by WB following IP.
Lane 1: ab95012 with 10µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of Exonuclease 1
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (4)
ab95012 は 4 報の論文で使用されています。
- Yan S et al. ZGRF1 promotes end resection of DNA homologous recombination via forming complex with BRCA1/EXO1. Cell Death Discov 7:260 (2021). PubMed: 34552057
- Yang G et al. EXO1 Plays a Carcinogenic Role in Hepatocellular Carcinoma and is related to the regulation of FOXP3. J Cancer 11:4917-4932 (2020). PubMed: 32626539
- Xie Y et al. RBX1 prompts degradation of EXO1 to limit the homologous recombination pathway of DNA double-strand break repair in G1 phase. Cell Death Differ 27:1383-1397 (2020). PubMed: 31562368
- Lee KY et al. MCM8-9 complex promotes resection of double-strand break ends by MRE11-RAD50-NBS1 complex. Nat Commun 6:7744 (2015). WB . PubMed: 26215093