Anti-EVI1 抗体 [EPR5996] (ab124934)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5996] to EVI1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-EVI1 antibody [EPR5996]
EVI1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5996] to EVI1 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,IHC-P or IP -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide within Human EVI1 aa 300-400. The exact sequence is proprietary.
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ポジティブ・コントロール
- HeLa and SKOV 3 cell lysates
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA disodium salt, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5996 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab124934の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 200, 145, 105 kDa (predicted molecular weight: 118 kDa).
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 200, 145, 105 kDa (predicted molecular weight: 118 kDa). |
ターゲット情報
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関連性
EVI1 promotes cell proliferation by interacting with BRG1 and blocking the repression of BRG1 on E2F1 activity. EVI1 may be involved in megakaryocytic differentiation. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 2122 Human
- Omim: 165215 Human
- SwissProt: Q03112 Human
- SwissProt: Q13465 Human
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別名
- AML1 EVI 1 antibody
- AML1 EVI 1 fusion protein antibody
- AML1 EVI1 antibody
see all
画像
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All lanes : Anti-EVI1 antibody [EPR5996] (ab124934) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SKOV 3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti Rabbit at 1/2000 dilution
Predicted band size: 118 kDa
Observed band size: 145,200 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible cleavage fragment)
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab124934 は 3 報の論文で使用されています。
- Luciani GM et al. Characterization of inv(3) cell line OCI-AML-20 with stroma-dependent CD34 expression. Exp Hematol 69:27-36 (2019). PubMed: 30352278
- Ma H et al. PBK, targeted by EVI1, promotes metastasis and confers cisplatin resistance through inducing autophagy in high-grade serous ovarian carcinoma. Cell Death Dis 10:166 (2019). PubMed: 30778048
- Ivanochko D et al. Direct interaction between the PRDM3 and PRDM16 tumor suppressors and the NuRD chromatin remodeling complex. Nucleic Acids Res N/A:N/A (2018). PubMed: 30462309