Anti-ETFB 抗体 (ab104944)
Key features and details
- Goat polyclonal to ETFB
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ETFB antibody
ETFB 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to ETFB -
由来種
Goat -
特異性
ab104944 is expected to recognize both reported isoforms (NP_001976.1; NP_001014763.1). -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Cow, Dog, Pig
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免疫原
Synthetic peptide corresponding to Human ETFB aa 150-250 (internal sequence). NP_001976.1
Database link: P38117 -
ポジティブ･コントロール
- Human, Mouse and Rat Liver lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab104944 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104944の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa).
1 hour primary incubation is recommended for this product. |
|
| IHC-P |
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 28 kDa). 1 hour primary incubation is recommended for this product. |
|
IHC-P
Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). -
組織特異性
Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas. -
関連疾患
Glutaric aciduria 2B -
配列類似性
Belongs to the ETF beta-subunit/FixA family. -
ドメイン
The recognition loop recognizes a hydrophobic patch at the surface of interacting dehydrogenases and acts as a static anchor at the interface. -
翻訳後修飾
Methylated. Trimethylation at Lys-200 and Lys-203 may negatively regulate the activity in electron transfer from Acyl-CoA dehydrogenases. -
細胞内局在
Mitochondrion matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 617210 Cow
- Entrez Gene: 2109 Human
- Entrez Gene: 396614 Pig
- Omim: 130410 Human
- SwissProt: Q2TBV3 Cow
- SwissProt: P38117 Human
- SwissProt: Q6UAQ8 Pig
- Unigene: 348531 Human
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別名
- Beta ETF antibody
- Beta-ETF antibody
- Electron transfer flavoprotein beta polypeptide antibody
see all
画像
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Western blot - Anti-ETFB antibody (ab104944)Anti-ETFB antibody (ab104944) at 0.1 µg/ml + Human Liver lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 28 kDa
Primary incubation was 1 hour.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab104944 は 2 報の論文で使用されています。
- Zhao Q et al. Screening and identification of the differentially expressed proteins in neonatal rat kidney after partial unilateral ureteral obstruction. Mol Med Rep 14:681-8 (2016). WB ; Rat . PubMed: 27222353
- Stauch KL et al. Loss of Pink1 modulates synaptic mitochondrial bioenergetics in the rat striatum prior to motor symptoms: concomitant complex I respiratory defects and increased complex II-mediated respiration. Proteomics Clin Appl 10:1205-1217 (2016). PubMed: 27568932
