Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Posterior part of an E9.5 embryo)
Permeabilization
Yes - 0.01% Tween-20, 0.1% Triton-X in PBS
Specification
Posterior part of an E9.5 embryo
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% serum 0.01% Tween-20, 0.1% Triton-X
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: AlexaFluor donkey anti-rabbit 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Abcam response
Thank you for sharing this data with us. ab19331 has previously been shown to work in IHC-Fr on rat tissue, please see Collin et al., 2008. However, based on the data shown here, this antibody either does not work in IHC-Fr, or on mouse, or both, under these experimental conditions. We would welcome feedback for other researchers currently using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Malgorzata Borkowska
Verified customer
投稿 Jul 24 2019