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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Estrogen Related Receptor alpha 抗体 [EPR46Y] (ab76228)

Reviews (1) Submit a question References (32)
Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
  • Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
  • Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
  • Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
  • Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
  • Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha
  • Suitable for: WB, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Estrogen Related Receptor alpha antibody [EPR46Y]
    Estrogen Related Receptor alpha 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR46Y] to Estrogen Related Receptor alpha

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IP, Flow Cytmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide within Human Estrogen Related Receptor alpha aa 1-100 (N terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76228の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IP
1/20.
Flow Cyt
1/20.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
IP
1/20.
Flow Cyt
1/20.

ターゲット情報

  • 機能

    Binds to an ERR-alpha response element (ERRE) containing a single consensus half-site, 5'-TNAAGGTCA-3'. Can bind to the medium-chain acyl coenzyme A dehydrogenase (MCAD) response element NRRE-1 and may act as an important regulator of MCAD promoter. Binds to the C1 region of the lactoferrin gene promoter. Requires dimerization and the coactivator, PGC-1A, for full activity. The ERRalpha/PGC1alpha complex is a regulator of energy metabolism.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • 翻訳後修飾

    Phosphorylation on Ser-19 enhances sumoylation on Lys-14 increasing repression of transcriptional activity.
    Sumoylated by SUMO2. Main site is Lys-14 which is enhanced by phosphorylation on Ser-19, cofactor activation, and by interaction with PIAS4. Sumoylation enhances repression of transcriptional activiy, but has no effect on subcellular location nor on DNA binding.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Err 1 antibody
    • ERR a antibody
    • ERR alpha antibody
    • ERR-alpha antibody
    • Err1 antibody
    • ERR1 protein antibody
    • ERR1_HUMAN antibody
    • ERRa antibody
    • ERRalpha antibody
    • ESRL 1 antibody
    • ESRL1 antibody
    • ESRR A antibody
    • Esrra antibody
    • Estrogen receptor like 1 antibody
    • Estrogen receptor related 1 antibody
    • estrogen receptor related receptor alpha antibody
    • Estrogen receptor-like 1 antibody
    • Estrogen related receptor alpha antibody
    • Estrogen-related receptor alpha antibody
    • Estrra antibody
    • hERR1 antibody
    • NR3B1 antibody
    • Nuclear receptor subfamily 3 group B member 1 antibody
    • Steroid hormone receptor ERR1 antibody
    see all

画像

  • Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
    Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
    All lanes : Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified)

    Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
    Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
    Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228) at 1/1000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 55 kDa
    Observed band size: 55 kDa

  • Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
    Immunoprecipitation - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

    ab76228 used at 1:20 dilution (2μg) immunoprecipitating Estrogen Related Receptor alpha in Hela whole cell lysates.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg.
    Lane 2 (+): HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab190685 in HeLa whole cell lysate.

    For western blotting, ab227648 and VeriBlot for IP Detection Reagent (HRP)(ab131366) at 1/1000 dilution were used.

  • Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
    Western blot - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Estrogen Related Receptor alpha knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: MCF7 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab76228 observed at 55 kDa. Red - loading control, ab18058, observed at 130 kDa.

    ab76228 was shown to specifically react with Estrogen Related Receptor alpha in wild-type HAP1 cells as signal was lost in Estrogen Related Receptor alpha knockout cells. Wild-type and Estrogen Related Receptor alpha knockout samples were subjected to SDS-PAGE. Ab76228 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
    Flow Cytometry - Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

    Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Estrogen Receptor alpha with ab76228 at 1/20 dilution (10 µg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)
    Anti-Estrogen Related Receptor alpha antibody [EPR46Y] (ab76228)

参考文献 (32)

ab76228 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76228 は 32 報の論文で使用されています。

  • Vangrieken P  et al. Hypoxia-induced mitochondrial abnormalities in cells of the placenta. PLoS One 16:e0245155 (2021). PubMed: 33434211
  • Yan H  et al. Adeno-associated virus-mediated delivery of anti-miR-199a tough decoys attenuates cardiac hypertrophy by targeting PGC-1alpha. Mol Ther Nucleic Acids 23:406-417 (2021). PubMed: 33473326
  • Li X  et al. ERRa activates SHMT2 transcription to enhance the resistance of breast cancer to lapatinib via modulating the mitochondrial metabolic adaption. Biosci Rep 40:N/A (2020). PubMed: 31894856
  • Xu Z  et al. Nuclear receptor ERRa contributes to castration-resistant growth of prostate cancer via its regulation of intratumoral androgen biosynthesis. Theranostics 10:4201-4216 (2020). PubMed: 32226548
  • Oka SI  et al. Perm1 regulates cardiac energetics as a downstream target of the histone methyltransferase Smyd1. PLoS One 15:e0234913 (2020). PubMed: 32574189
View all Publications for this product

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Western blot abreview for Anti-Estrogen Related Receptor alpha antibody [EPR46Y]

 Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (mammary epithelial)
Loading amount
80 µg
Specification
mammary epithelial
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
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投稿 Jun 25 2009

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