Anti-Estrogen Receptor beta 抗体 [14C8] - BSA and Azide free (ab288)
![Western blot - Anti-Estrogen Receptor beta antibody [14C8] - BSA and Azide free (ab288)](https://www.abcam.co.jp/ps/products/0/ab288/Images/ab288-393596-AntiEstrogen-Receptor-beta-antibody-14C8-westernblot-HEK293T-whole-cell-extracts.JPG "Western blot - Anti-Estrogen Receptor beta antibody [14C8] - BSA and Azide free (ab288)")
Key features and details
- Mouse monoclonal [14C8] to Estrogen Receptor beta - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-Estrogen Receptor beta antibody [14C8] - BSA and Azide free
Estrogen Receptor beta 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [14C8] to Estrogen Receptor beta - BSA and Azide free -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human Estrogen Receptor beta aa 1-153. Expressed in E.coli.
Database link: Q92731 -
ポジティブ･コントロール
- WB: Recombinant fusion protein, human liver and intestine, HEK-293T cells.
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特記事項
This product was changed from ascites to tissue culture supernatant on 23rd October 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: 100% PBS -
キャリア・フリー
はい -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
14C8 -
ミエローマ
NS1 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab288の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa.
Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight. |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight. |
ターゲット情報
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機能
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. -
組織特異性
Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 2100 Human
- Omim: 601663 Human
- SwissProt: Q92731 Human
- Unigene: 660607 Human
- Unigene: 729020 Human
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別名
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
画像
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Western blot - Anti-Estrogen Receptor beta antibody [14C8] - BSA and Azide free (ab288)All lanes : Anti-Estrogen Receptor beta antibody [14C8] - BSA and Azide free (ab288) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : Transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5% SDS-PAGE
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![Western blot - Anti-Estrogen Receptor beta antibody [14C8] - BSA and Azide free (ab288)](https://www.abcam.co.jp/ps/products/0/ab288/Images/ab288-348988-anti-estrogen-receptor-beta-antibody-14c8-western-blot-human-brain-liver-intestine.jpg)
Western blot - Anti-Estrogen Receptor beta antibody [14C8] - BSA and Azide free (ab288)All lanes : Anti-Estrogen Receptor beta antibody [14C8] - BSA and Azide free (ab288) at 1/500 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human intestine tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Mouse IgG antibody (HRP) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 59 kDa7.5% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition: Semi-dry, 18 V, 60min (NC membrane)
blocking condition: 5% non-fat milk in TBST, RT, 60min.
primary antibody incubation: 4°C overnight.
Secondary antibody incubation: room temperature for 1 hour.
Washing conditions: 5 ml TBST, 4 x 5min.
ECL exposure.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (88)
ab288 は 88 報の論文で使用されています。
- Sun Z et al. 17ß-Estradiol promotes LC3B-associated phagocytosis in trained immunity of female mice against sepsis. Int J Biol Sci 17:460-474 (2021). PubMed: 33613105
- Gao J et al. Risk factors predicting the occurrence of metachronous ovarian metastasis of gastric cancer. Ann Transl Med 9:1049 (2021). PubMed: 34422961
- Gu J et al. Targeting the ERß/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC. Cell Death Dis 11:367 (2020). PubMed: 32409702
- Li H et al. CLPTM1L induces estrogen receptor ß signaling-mediated radioresistance in non-small cell lung cancer cells. Cell Commun Signal 18:152 (2020). PubMed: 32943060
- Zhang E et al. Apigenin Inhibits Histamine-Induced Cervical Cancer Tumor Growth by Regulating Estrogen Receptor Expression. Molecules 25:N/A (2020). PubMed: 32340124