Anti-Estrogen Receptor beta 抗体 [14C8] (ab288)
Key features and details
- Mouse monoclonal [14C8] to Estrogen Receptor beta
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Monkey
- Isotype: IgG2b
製品の概要
-
製品名
Anti-Estrogen Receptor beta antibody [14C8]
Estrogen Receptor beta 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [14C8] to Estrogen Receptor beta -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human, Monkey -
免疫原
Recombinant fragment corresponding to Human Estrogen Receptor beta aa 1-153. Expressed in E.coli.
Database link: Q92731 -
ポジティブ・コントロール
- WB: Recombinant fusion protein, human liver and intestine, HEK-293T cells. IHC-P: Endocervical epithelium, Lobular breast carcinoma and human breast carcinoma.
-
特記事項
This product was changed from ascites to tissue culture supernatant on 23rd October 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
14C8 -
ミエローマ
NS1 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
-
ChIP Related Products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab288 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 59 kDa. | |
IHC-P | Use a concentration of 5 µg/ml. |
ターゲット情報
-
機能
Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. -
組織特異性
Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Omim: 601663 Human
- SwissProt: Q92731 Human
- SwissProt: O08537 Mouse
- Unigene: 660607 Human
- Unigene: 729020 Human
- Unigene: 2561 Mouse
-
別名
- ER BETA antibody
- ER-beta antibody
- Erb antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)
Paraffin embedded human breast carcinoma tissue stained for Estrogen Receptor beta using ab288 at 1/200 dilution in immunohistochemical analysis.
-
All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/5000 dilution
Lane 1 : Non-transfected HEK-293T whole cell extracts
Lane 2 : Transfected HEK-293T whole cell extracts
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa7.5% SDS-PAGE
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)
ab288 staining Estrogen Receptor beta in infiltrating lobular carcinoma of the human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde. Endogenous peroxidase blocking was with 3% H2O2 at room temperature for 30 minutes. Blocking with 1.5% goat serum (dilute goat serum by 1xPBS), at room temperature for 30min; antigen retrieval was by heat mediation in a citrate buffer, pH 6.0, 15min (Cuisinart Electric Pressure Cooker #EPC-1200, choose "high pressure"). Samples were incubated with primary antibody (1/100) for overnight at 4°C. An ABC HRP Kit Mouse IgG) was used as the secondary antibody (1/200), room temperature for 30min. Washed twice for 5 minutes using PBS. DAB was used as a substrate.
-
All lanes : Anti-Estrogen Receptor beta antibody [14C8] (ab288) at 1/500 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human liver tissue lysate
Lane 3 : Human intestine tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Mouse IgG antibody (HRP) at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 59 kDa7.5% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition: Semi-dry, 18 V, 60min (NC membrane)
blocking condition: 5% non-fat milk in TBST, RT, 60min.
primary antibody incubation: 4°C overnight.
Secondary antibody incubation: room temperature for 1 hour.
Washing conditions: 5 ml TBST, 4 x 5min.
ECL exposure.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor beta antibody [14C8] (ab288)ab288 staining endocervical epithelium by IHC-P.
プロトコール
参考文献 (79)
ab288 は 79 報の論文で使用されています。
- Gu J et al. Targeting the ERß/Angiopoietin-2/Tie-2 signaling-mediated angiogenesis with the FDA-approved anti-estrogen Faslodex to increase the Sunitinib sensitivity in RCC. Cell Death Dis 11:367 (2020). PubMed: 32409702
- Li H et al. CLPTM1L induces estrogen receptor ß signaling-mediated radioresistance in non-small cell lung cancer cells. Cell Commun Signal 18:152 (2020). PubMed: 32943060
- Song P et al. Estrogen receptor ß inhibits breast cancer cells migration and invasion through CLDN6-mediated autophagy. J Exp Clin Cancer Res 38:354 (2019). PubMed: 31412908
- Datta N et al. Promyelocytic Leukemia (PML) gene regulation: implication towards curbing oncogenesis. Cell Death Dis 10:656 (2019). PubMed: 31506431
- López-Romero R et al. The expression of transcription factor BORIS and its association with the estrogen receptor beta (ER-ß) in cervical carcinogenesis. Int J Clin Exp Pathol 12:3208-3221 (2019). PubMed: 31934165