Anti-Estrogen Receptor beta 1 抗体 [ERb455] - C-terminal (ab187291)
Key features and details
- Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Sheep, Horse, Human, Pig, Monkey
- Isotype: IgG2a
Related conjugates and formulations
製品の概要
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製品名
Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal -
製品の詳細
Mouse monoclonal [ERb455] to Estrogen Receptor beta 1 - C-terminal -
由来種
Mouse -
特異性
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ER-alpha and ER-beta, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ER-alpha and ER-beta are differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ER-alpha and ER-beta may be regulated by distinct mechanisms even though they share many functional characteristics. -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Sheep, Horse, Human, Pig, Monkey -
免疫原
Recombinant fragment corresponding to Human Estrogen Receptor beta 1 (C terminal).
Database link: Q92731 -
ポジティブ・コントロール
- Flow Cyt: BT474 and MCF-7 cells IHC-P: Ovarian carcinoma, breast carcinoma tissues, gastric carcinoma, and bladder carcinoma tissues. WB: MCF7 cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
ab187291 was purified from Bioreactor concentrate. -
ポリ/モノ
モノクローナル -
クローン名
ERb455 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab187291の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa.
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IHC-P | (6) |
Use a concentration of 1 - 2 µg/ml.
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
Flow Cyt |
Use 1-2µg for 106 cells.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 59 kDa. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes. |
Flow Cyt
Use 1-2µg for 106 cells. |
ターゲット情報
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関連性
This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 100033964 Horse
- Entrez Gene: 2100 Human
- Entrez Gene: 13983 Mouse
- Entrez Gene: 396697 Pig
- Entrez Gene: 25149 Rat
- Entrez Gene: 443066 Sheep
- Omim: 601663 Human
- SwissProt: Q92731 Human
see all -
別名
- ER-BETA antibody
- Erb antibody
- ESR-BETA antibody
see all
画像
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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Anti-Estrogen Receptor beta 1 antibody [ERb455] - C-terminal (ab187291) at 2 µg/ml + MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Predicted band size: 59 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast cancer tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Gastric Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human Bladder Carcinoma tissue labeling Estrogen Receptor beta 1 with ab187291 at 1 µg/ml.
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Flow cytometry analysis of BT474 (human ductal breast epithelial tumor cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Grey-isotype control,Green-AF4888-labeled ER beta 1.
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Flow cytometry analysis of MCF-7 (human breast adenocarcinoma cell line) cells labelling ER beta with ab187291 at 0.5 ug/ml. Black-cells alone, Green-isotype control, Red-PE labeled ER-beta 1.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab187291 は 4 報の論文で使用されています。
- Xu Z et al. Positive interaction between GPER and β-alanine in the dorsal root ganglion uncovers potential mechanisms: mediating continuous neuronal sensitization and neuroinflammation responses in neuropathic pain. J Neuroinflammation 19:164 (2022). PubMed: 35729568
- Padma AM et al. Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioning. NPJ Regen Med 6:26 (2021). PubMed: 34021161
- Tiemann TT et al. Towards uterus tissue engineering: a comparative study of sheep uterus decellularisation. Mol Hum Reprod 26:167-178 (2020). PubMed: 31980817
- Zhang L et al. Estrogen Receptor ß1 Expression Patterns Have Different Effects on Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors' Treatment Response in Epidermal Growth Factor Receptor Mutant Lung Adenocarcinoma. Front Oncol 10:603883 (2020). PubMed: 33585221