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Anti-Estrogen Receptor alpha 抗体 [TE111.5D11] (ab16460)

Reviews (1)Q&A (6)References (10)
Western blot - Anti-Estrogen Receptor alpha antibody [TE111.5D11] (ab16460)

    Key features and details

    • Mouse monoclonal [TE111.5D11] to Estrogen Receptor alpha
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-Estrogen Receptor alpha antibody [TE111.5D11]
      Estrogen Receptor alpha 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [TE111.5D11] to Estrogen Receptor alpha

    • 由来種

      Mouse

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Chicken, Hamster, Cow, Dog, Pig

    • 免疫原

      Recombinant fragment corresponding to Human Estrogen Receptor alpha. Recombinant fragment, corresponding to amino acids 302-595 of Human Estrogen Receptor alpha.
      Database link: P03372

    • ポジティブ･コントロール

      • WB and Flow Cyt: MCF-7 cells.

    • 特記事項

      The estrogen receptor (ER) gene consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains required for transcription activation function, binding to estrogen response element (ERE) constitutive dimerization, binding to heat shock proteins, and ligand recognition. The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab16460の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (1)
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).
    特記事項
    WB
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 66 kDa).

    ターゲット情報

    • 機能

      Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.

    • 配列類似性

      Belongs to the nuclear hormone receptor family. NR3 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.

    • ドメイン

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

    • 翻訳後修飾

      Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      Glycosylated; contains N-acetylglucosamine, probably O-linked.
      Ubiquitinated. Deubiquitinated by OTUB1.
      Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
      Palmitoylated (isoform 3). Not biotinylated (isoform 3).

    • 細胞内局在

      Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • 7*/654 isoform antibody
      • 7*/819 2 isoform antibody
      • 7*/822 isoform antibody
      • 8*/901 isoform antibody
      • 8*/941 isoform antibody
      • DKFZp686N23123 antibody
      • ER alpha antibody
      • ER antibody
      • ER-alpha antibody
      • Era antibody
      • ESR antibody
      • ESR1 antibody
      • ESR1_HUMAN antibody
      • ESRA antibody
      • Estradiol receptor antibody
      • Estrogen nuclear receptor alpha antibody
      • Estrogen receptor 1 antibody
      • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
      • Estrogen receptor alpha antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
      • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
      • Estrogen receptor alpha delta 4 +49 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
      • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
      • Estrogen receptor alpha E1 E2 1 2 antibody
      • Estrogen receptor alpha E1 N2 E2 1 2 antibody
      • Estrogen receptor antibody
      • ESTRR antibody
      • NR3A1 antibody
      • Nuclear receptor subfamily 3 group A member 1 antibody
      see all

    画像

    • Western blot - Anti-Estrogen Receptor alpha antibody [TE111.5D11] (ab16460)
      Western blot - Anti-Estrogen Receptor alpha antibody [TE111.5D11] (ab16460)

      ab16460 at 1µg/ml detecting Estrogen Recptor from MCF-7 cells by Western blot.

    参考文献 (10)

    ab16460 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab16460 は 10 報の論文で使用されています。

    • Lin ZH  et al. The effects of estradiol on inflammatory and endothelial dysfunction in rats with preeclampsia. Int J Mol Med 45:825-835 (2020). PubMed: 31985028
    • Majorini MT  et al. Infiltrating Mast Cell-Mediated Stimulation of Estrogen Receptor Activity in Breast Cancer Cells Promotes the Luminal Phenotype. Cancer Res 80:2311-2324 (2020). PubMed: 32179512
    • Wang B  et al. A dual role of miR-22 modulated by RelA/p65 in resensitizing fulvestrant-resistant breast cancer cells to fulvestrant by targeting FOXP1 and HDAC4 and constitutive acetylation of p53 at Lys382. Oncogenesis 7:54 (2018). PubMed: 30057418
    • Cho KA  et al. Mesenchymal stem cells ameliorate B-cell-mediated immune responses and increase IL-10-expressing regulatory B cells in an EBI3-dependent manner. Cell Mol Immunol N/A:N/A (2017). PubMed: 28042143
    • Deng X  et al. Guanxin Danshen Formulation Protects against Myocardial Ischemia Reperfusion Injury-Induced Left Ventricular Remodeling by Upregulating Estrogen Receptor ß. Front Pharmacol 8:777 (2017). PubMed: 29163163
    • Wan Abdul Rahman WF  et al. Expression of DNA methylation marker of paired-like homeodomain transcription factor 2 and growth receptors in invasive ductal carcinoma of the breast. Asian Pac J Cancer Prev 15:8441-5 (2014). PubMed: 25339043
    • Elbaradie KB  et al. Sex-specific response of rat costochondral cartilage growth plate chondrocytes to 17ß-estradiol involves differential regulation of plasma membrane associated estrogen receptors. Biochim Biophys Acta 1833:1165-72 (2013). PubMed: 23305904
    • Marmugi A  et al. Low doses of bisphenol A induce gene expression related to lipid synthesis and trigger triglyceride accumulation in adult mouse liver. Hepatology 55:395-407 (2012). PubMed: 21932408
    • Pedram A  et al. Developmental phenotype of a membrane only estrogen receptor alpha (MOER) mouse. J Biol Chem 284:3488-95 (2009). PubMed: 19054762
    • Safdar A  et al. miRNA in the regulation of skeletal muscle adaptation to acute endurance exercise in C57Bl/6J male mice. PLoS One 4:e5610 (2009). WB ; Human . PubMed: 19440340

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Estrogen Receptor antibody [TE111.5D11]

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MCF7, Monocytes and Monocyte-Derived-Macrophages)
    Loading amount
    25 µg
    Specification
    MCF7, Monocytes and Monocyte-Derived-Macrophages
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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    Abcam user community

    Verified customer

    投稿 Apr 16 2008

    Question

    Estamada ,
    Quería saber si sería tan amable de indicarme la concentración a la cuál emplean este anticuerpo con esa actividad bloqueante, ya que lo han testado en su compañía.
    Muchas gracias de antemano. Un saludo,

    Read More
    Answer

    Gracias por tu respuesta.

    No hay ninguna recomendación específica acerca de la concentración usada como bloqueante para este anticuerpo. La concentración usada depende totalmente de las condiciones experimentales, y debe ser calculada por el usuario.

    Siento no poder ser de más ayuda. Si tienes cualquier otra consulta, por favor, no dudes en contactarme de nuevo.

    Read More

    Question

    ab16460:
    Human Estrogen Receptor
    alpha or beta?

    Read More
    Answer

    Thank you for your call.

    I can confirm that immunogen for this antibody was a recombinant fragment corresponding to amino acids 302-595 of Human Estrogen Receptor alpha.

    This sequence show a 55% homology to Human Estrogen Receptor beta and we therefore would not expect a cross reaction of the antibody. But this was not experimentally tested and therefore is not guaranteed.

    I hope this information is helpful and wish you a good weekend.

    Read More

    Question

    do you know if these aa correspond to beta estradiol?

    Read More
    Answer

    Thank you for your reply.
    I am not sure if these amino acids correspond with beta estradiol, however the immunogen that was used to generate the antibody is a recombinant fragment, corresponding to amino acids 302-595 of Human Estrogen Receptor. Therefore you could align this section of the human estrogen receptor with your target sequence, and see the level of similarity.
    Please let me know if there is anything else I can help you with.

    Read More

    Question

    Greetings, Anti-Estrogen Receptor antibody [TE111.5D11] (ab16460) Is this antibody against the receptor that binds beta estradiol? thank you.

    Read More
    Answer

    Thank you for contacting Abcam.
    The antibody ab16460 will bind to the estorgen receptor and the immunogen is a recombinant fragment, corresponding to amino acids 302-595 of Human Estrogen Receptor.
    If there is anything else I can help you with, please let me know.

    Read More

    Question

    This customer has already purchased ab16460 (Anti-Estrogen Receptor antibody [TE111.5D11]), and after he conducted western blot assay, the result shows multiple bands and wrong band size, therefore he wants to ask for you help to determine the correct size of her western blot image, I attached her wb data in this letter, please help this customer to solve the problem? Thanks for your assistance.       

    Read More
    Answer

    Thank you for sending the customer's western blot image. I am having difficulty reading it, and seeing where the signal is detected in the blots. I understand that the customer expects to see the receptor at 65 kDa, but does not. Can you please ask the customer: 1. What are the samples (species, cell types)? How are they prepared (RIPA lysis buffer?) and are they collected with protease inhibitors? Does the customer boil the samples to denature, with a reducing agent? 2. How are the membranes blocked? With 5% milk? 3. What is the dilution or concentration of the antibody that the customer applies tot he membrane? 4. What are the sizes (molecular weights) of the bands that the customer does detect? I look forward to resolving this issue for your customer.

    Read More

    Question

    so could you pls indicate which antibody I should buy which is ER alpha specific for western blotting and does definitely not cross react with the beta form. Also you did not answer my question regarding the different antibodies you have and the question for the actin

    Read More
    Answer

    Thank you for your enquiry. I would like to recommend the following ER alpha antibody; Estrogen Receptor alpha antibody [33] (ab2746). we have good feedback regarding the behaviour of this antibody by western blotting. It is also a monoclonal antibody. We do not have any knowledge of non-specific reactivity towards ER beta. I have also performed an alignment of the immunogen used with the ER beta sequence and there is only very minimal sequence similarity suggesting that this antibody would not show this kind of reactivity. With regards beta Actin antibody [mAbcam 8226] - Loading Control (ab8226) we do not have specific details of MCF7 detection by this antibody. However, I would certainly expect it to detect beta actin in this cell type using an HRP conjugated secondary antibody. Furthermore we would guarantee it for this purpose. Please see our "Abpromise". I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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