Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (stable Sirt-3 overexpressing MCF-7 breast cancer c)
Permeabilization
Yes - 0.1% Triton X
Specification
stable Sirt-3 overexpressing MCF-7 breast cancer c
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: +4°C · Diluent: 1% BSA + 0.1% Triton X + PBS (pH=7.4)
Dilution
1/500
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat-Anti Rabbit IgG (H+L) Cross-Adsorbed
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sandra Sobocanec
Verified customer
投稿 Jan 15 2019