Anti-Estrogen Receptor alpha 抗体 [SP1] (ab16660)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP1] to Estrogen Receptor alpha
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Estrogen Receptor alpha antibody [SP1]
Estrogen Receptor alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP1] to Estrogen Receptor alpha -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
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エピトープ
C-terminal -
ポジティブ・コントロール
- WB: MCF7 cell lysate. IHC-P: Human breast carcinoma, cervix, breast, breast ductal carcinoma and ovarian adenocarcinoma tissue. ICC/IF: MCF7 cells. Flow Cyt (intra): MCF7 cells.
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特記事項
This product has switched from a hybridoma to recombinant production format on 21st May 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
SP1 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab16660の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/200.
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WB |
1/25. Predicted molecular weight: 67 kDa.
Incubate for 1 hour at room temperature. |
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IHC-P | (1) |
1/200.
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ICC/IF | (1) |
1/25 - 1/250.
|
特記事項 |
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Flow Cyt (Intra)
1/200. |
WB
1/25. Predicted molecular weight: 67 kDa. Incubate for 1 hour at room temperature. |
IHC-P
1/200. |
ICC/IF
1/25 - 1/250. |
ターゲット情報
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機能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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参照データベース
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Omim: 133430 Human
- SwissProt: P03372 Human
- SwissProt: P19785 Mouse
- Unigene: 208124 Human
- Unigene: 463262 Mouse
- Unigene: 9213 Mouse
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別名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
IHC image of ab16660 staining Estrogen Receptor alpha in normal human cervix formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16660, 1/250 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
This image was generated using the hybridoma version of the product.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ab16660 staining Estrogen Receptor alpha in MCF7 cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab16660 at 1/250 dilution (shown in green) and ab195889, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 594), at 2µg/ml (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).This image was generated using the hybridoma version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) at 1/25 dilution + lysate prepared from MCF7 cells
Predicted band size: 67 kDaThis image was generated using the hybridoma version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)Image courtesy of Joseph J D et al. eLife 2016;5:e15828 doi: 10.7554/eLife.15828
Immunohistochemical analysis of tamoxifen resistant MCF7 xenograft tumours staining estrogen receptor alpha with ab16660. The mice used were treated with a vehicle, tamoxifen (120mg/kg/day p.o) or GDC-0810 (100mg/kg/day p.o.) for 27 days.
This image was generated using the hybridoma version of the product.
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Intracellular flow cytometry analysis of MCF7 (human breast adenocarcinoma epithelial cell) labeling Estrogen Receptor alpha with purified ab16660 at 1/200 dilution (1.06 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue). This image was generated using the hybridoma version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling Estrogen Receptor alpha with purified ab16660 at 1/25 (8.5 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This image was generated using the hybridoma version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
Formalin-fixed, paraffin-embedded human breast tissue stained for Estrogen Receptor alpha using ab16660 at 1/200 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
Human breast carcinoma stained with ab16660.
This image was generated using the hybridoma version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (50)
ab16660 は 50 報の論文で使用されています。
- Weng Y et al. miR-133b has protective effect on rats with acute lung injury caused by severe acute pancreatitis through targeting sp1 gene. Int J Clin Exp Pathol 14:86-96 (2021). PubMed: 33532026
- Li W et al. A synergetic effect of BARD1 mutations on tumorigenesis. Nat Commun 12:1243 (2021). PubMed: 33623049
- Kapara A et al. Detection of Estrogen Receptor Alpha and Assessment of Fulvestrant Activity in MCF-7 Tumor Spheroids Using Microfluidics and SERS. Anal Chem 93:5862-5871 (2021). PubMed: 33797884
- Kamiya Y et al. Puerarin blocks the aging phenotype in human dermal fibroblasts. PLoS One 16:e0249367 (2021). PubMed: 33886592
- Zhou H et al. Chaperone-mediated Autophagy Governs Progression of Papillary Thyroid Carcinoma via PPAR?-SDF1/CXCR4 Signaling. J Clin Endocrinol Metab 105:N/A (2020). PubMed: 32556197