Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (breast tumor xenograft in mouse)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization
No
Specification
breast tumor xenograft in mouse
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
10% normal buffered formalin
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/250
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP polymer)
Additional data
Abcam response
We would like to thank the reviewer for submitting this AbReview for ab108398. As this antibody has not yet been tested on murine samples we were unsure what the result might be and we appreciate the time and effort that the reviewer has put into testing this antibody on this species. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 24 2019