PE Anti-Estrogen Receptor alpha 抗体 [E115] (ab209288)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [E115] to Estrogen Receptor alpha
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
-
製品名
PE Anti-Estrogen Receptor alpha antibody [E115]
Estrogen Receptor alpha 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [E115] to Estrogen Receptor alpha -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
特異性
Expression levels of ER alpha protein vary with sample type. -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IFmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Dog -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ・コントロール
- Flow Cyt (Intra): T47D, 4T1 and GH3 cells. ICC/IF: T47D cells.
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
E115 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Assay kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab209288の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/50 - 1/2500.
|
|
ICC/IF |
1/500.
This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) |
特記事項 |
---|
Flow Cyt (Intra)
1/50 - 1/2500. |
ICC/IF
1/500. This product gave a positive signal in T47D cells fixed with 4% formaldehyde (10 min) |
ターゲット情報
-
機能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
-
参照データベース
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Entrez Gene: 24890 Rat
- Omim: 133430 Human
- SwissProt: P03372 Human
- SwissProt: P19785 Mouse
- SwissProt: P06211 Rat
- Unigene: 208124 Human
see all -
別名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
画像
-
ab209288 staining Estrogen Receptor alpha in T47D cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209288 at 1/500 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
-
Overlay histogram showing T47D cells stained with ab209288 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol for 30 min at -20°C. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209288, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
-
Intracellular Flow Cytometry analysis of 4T1 (mouse mammary gland carcinoma epithelial cell) cells labeling Estrogen Receptor alpha with ab209288 at 1/50 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. Isotype control - Rabbit IgG monoclonal [EPR25A, ab209478] (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
-
Intracellular Flow Cytometry analysis of GH3 (rat pituitary epithelial) cells labeling Estrogen Receptor alpha with ab209288 at 1/50 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. Isotype control - Rabbit IgG monoclonal [EPR25A, ab209478] (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
Certificate of Compliance
参考文献 (2)
ab209288 は 2 報の論文で使用されています。
- Zhao Y et al. Circulating Innate Lymphoid Cells Exhibit Distinctive Distribution During Normal Pregnancy. Reprod Sci 29:1124-1135 (2022). PubMed: 34988918
- Kim J et al. Chronic and Low Dose Exposure to Nonlyphenol or Di(2-Ethylhexyl) Phthalate Alters Cell Proliferation and the Localization of Steroid Hormone Receptors in Uterine Endometria in Mice. Dev Reprod 23:263-275 (2019). PubMed: 31660453