Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Hindlimb muscle)
Permeabilization
No
Specification
Hindlimb muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Acetone
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Additional data
Additional Notes
This antibody stained slow twitch muscle fibers in unfixed frozen rat muscle tissue. When frozen sections were fixed in ice cold acetone, cytoplasmic staining was reduced. Estrogen receptors were difficult to locate due to auto fluorescence of laminin in 488 channel.
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投稿 Jul 18 2019