Anti-Estrogen Receptor alpha 抗体 (ab3575)
Key features and details
- Rabbit polyclonal to Estrogen Receptor alpha
- Suitable for: ICC, IHC-P, WB
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Estrogen Receptor alpha antibody
Estrogen Receptor alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Estrogen Receptor alpha -
由来種
Rabbit -
アプリケーション
適用あり: ICC, IHC-P, WBmore details -
種交差性
交差種: Rat, Human, Recombinant fragment
交差が予測される動物種: Hamster, Cow, Cat
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免疫原
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ポジティブ･コントロール
- WB: recombinant fragment; IHC: rat prostate tissue; ICC: T47D and 578T breast cancer cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab3575の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
1/1000 - 1/4000.
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| IHC-P | (1) |
Use a concentration of 1 - 5 µg/ml.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847).
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| 特記事項 |
|---|
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ICC
1/1000 - 1/4000. |
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IHC-P
Use a concentration of 1 - 5 µg/ml. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847). |
ターゲット情報
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機能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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参照データベース
- Entrez Gene: 407238 Cow
- Entrez Gene: 101825539 Hamster
- Entrez Gene: 2099 Human
- Entrez Gene: 24890 Rat
- Omim: 133430 Human
- SwissProt: P49884 Cow
- SwissProt: Q9QZJ5 Hamster
- SwissProt: P03372 Human
see all -
別名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.
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Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution
Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDaBlocked with 5% milk in TBST for at least 1 hour at room temperature.
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Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab3575 は 17 報の論文で使用されています。
- Huang H et al. Down-regulation of ER-a36 mRNA in serum exosomes of the patients with hepatocellular carcinoma. Clin Transl Med 10:346-352 (2020). PubMed: 32508028
- Leffler KE & Abdel-Rahman AA Restoration of Adiponectin-Connexin43 Signaling Mitigates Myocardial Inflammation and Dysfunction in Diabetic Female Rats. J Cardiovasc Pharmacol 75:259-267 (2020). PubMed: 31868825
- Hernández-Vega AM et al. Estradiol Induces Epithelial to Mesenchymal Transition of Human Glioblastoma Cells. Cells 9:N/A (2020). PubMed: 32825553
- Kim CS et al. Tamoxifen ameliorates obstructive nephropathy through Src and the PI3K/Akt/mTOR pathway. Biol Cell 111:18-27 (2019). PubMed: 30304547
- Li Q et al. Estradiol promotes the progression of ER+ breast cancer through methylation-mediated RSK4 inactivation. Onco Targets Ther 12:5907-5916 (2019). PubMed: 31413588