Anti-Estrogen Receptor alpha 抗体 (ab3575)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
  • Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)
  • Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)

Key features and details

  • Rabbit polyclonal to Estrogen Receptor alpha
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Rat, Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Estrogen Receptor alpha antibody
    Estrogen Receptor alpha 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Estrogen Receptor alpha

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, IHC-P, WBmore details

  • 種交差性

    交差種: Rat, Human, Recombinant fragment
    交差が予測される動物種: Hamster, Cow, Cat

  • 免疫原

    Synthetic peptide corresponding to Human Estrogen Receptor alpha aa 21-32.
    Sequence:

    NELEPLNRPQLK


    Database link: P03372
    (Peptide available as ab5847)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: recombinant fragment; IHC: rat prostate tissue; ICC: T47D and 578T breast cancer cells

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3575の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
1/1000 - 1/4000.
IHC-P (1)
Use a concentration of 1 - 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847).
特記事項
ICC
1/1000 - 1/4000.
IHC-P
Use a concentration of 1 - 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847).

ターゲット情報

  • 機能

    Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR3 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 翻訳後修飾

    Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
    Glycosylated; contains N-acetylglucosamine, probably O-linked.
    Ubiquitinated. Deubiquitinated by OTUB1.
    Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
    Palmitoylated (isoform 3). Not biotinylated (isoform 3).

  • 細胞内局在

    Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • 7*/654 isoform antibody
    • 7*/819 2 isoform antibody
    • 7*/822 isoform antibody
    • 8*/901 isoform antibody
    • 8*/941 isoform antibody
    • DKFZp686N23123 antibody
    • ER alpha antibody
    • ER antibody
    • ER-alpha antibody
    • Era antibody
    • ESR antibody
    • ESR1 antibody
    • ESR1_HUMAN antibody
    • ESRA antibody
    • Estradiol receptor antibody
    • Estrogen nuclear receptor alpha antibody
    • Estrogen receptor 1 antibody
    • Estrogen receptor alpha 3*,4,5,6,7*/822 isoform antibody
    • Estrogen receptor alpha antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*,8*/941 isoform antibody
    • Estrogen receptor alpha delta 3*,4,5,6,7*/819 2 isoform antibody
    • Estrogen receptor alpha delta 4 +49 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7*/654 isoform antibody
    • Estrogen receptor alpha delta 4*,5,6,7,8*/901 isoform antibody
    • Estrogen receptor alpha E1 E2 1 2 antibody
    • Estrogen receptor alpha E1 N2 E2 1 2 antibody
    • Estrogen receptor antibody
    • ESTRR antibody
    • NR3A1 antibody
    • Nuclear receptor subfamily 3 group A member 1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)

    ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.

  • Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)
    Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)
    All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution

    Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
    Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
    Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
    Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa



    Blocked with 5% milk in TBST for at least 1 hour at room temperature.

  • Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)
    Immunocytochemistry - Anti-Estrogen Receptor alpha antibody (ab3575)

    4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.

参考文献 (15)

ab3575 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3575 は 15 報の論文で使用されています。

  • Huang H  et al. Down-regulation of ER-a36 mRNA in serum exosomes of the patients with hepatocellular carcinoma. Clin Transl Med 10:346-352 (2020). PubMed: 32508028
  • Kim CS  et al. Tamoxifen ameliorates obstructive nephropathy through Src and the PI3K/Akt/mTOR pathway. Biol Cell 111:18-27 (2019). PubMed: 30304547
  • Li Q  et al. Estradiol promotes the progression of ER+ breast cancer through methylation-mediated RSK4 inactivation. Onco Targets Ther 12:5907-5916 (2019). PubMed: 31413588
  • Liu YR  et al. ER-depletion lowering the 'hypothalamus-uterus-kidney' axis functions by perturbing the renal ERß/Ptgds signalling pathway. Aging (Albany NY) 11:9500-9529 (2019). PubMed: 31708494
  • Niranjan MK & Srivastava R Expression of estrogen receptor alpha in developing brain, ovary and shell gland of Gallus gallus domesticus: Impact of stress and estrogen. Steroids 146:21-33 (2019). PubMed: 30885650
View all Publications for this product

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1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Estrogen Receptor alpha antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Hindlimb muscle)
Permeabilization
No
Specification
Hindlimb muscle
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Acetone
Read More

Abcam user community

Verified customer

投稿 Jul 18 2019

ChIP abreview for Anti-Estrogen Receptor alpha antibody

 Excellent
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (MCF7 breast cancer cell line and tumour samples)
Specification
MCF7 breast cancer cell line and tumour samples
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: Disuccimidyl glutarate (20 min
Positive control
Histone acetylation ChIP-seq was used as positive control
Read More

Dr. Sankari Nagarajan

Verified customer

投稿 Apr 01 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Estrogen Receptor alpha antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Breast Cancer Metastasis in Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ph6 citrate
Permeabilization
No
Specification
Breast Cancer Metastasis in Lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Dr. Victoria Bridgeman

Verified customer

投稿 May 13 2016

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