Anti-Estrogen Receptor alpha 抗体 (ab3575)
製品の概要
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製品名
Anti-Estrogen Receptor alpha antibody
Estrogen Receptor alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Estrogen Receptor alpha -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, Flow Cyt, ICC, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human, Pig, Deer
交差が予測される動物種: Hamster, Cow, Cat
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免疫原
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ポジティブ･コントロール
- ICC: Human coronary artery smooth muscle cells (See Han et al). IHC-P: Rat prostate tissue. ICC/IF: T-47D cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human breast ductal carcinoma
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab3575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 0.5 mg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 68 kDa).Can be blocked with Human Estrogen Receptor alpha peptide (ab5847). | |
| Flow Cyt | Use at an assay dependent concentration. PubMed: 23768049 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| ICC | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能
Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1. -
配列類似性
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
翻訳後修飾
Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
Glycosylated; contains N-acetylglucosamine, probably O-linked.
Ubiquitinated. Deubiquitinated by OTUB1.
Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization.
Palmitoylated (isoform 3). Not biotinylated (isoform 3). -
細胞内局在
Nucleus. Cytoplasm. Cell membrane. A minor fraction is associated with the inner membrane and Nucleus. Cytoplasm. Cell membrane. Associated with the inner membrane via palmitoylation. - Information by UniProt
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参照データベース
- Entrez Gene: 407238 Cow
- Entrez Gene: 101825539 Hamster
- Entrez Gene: 2099 Human
- Entrez Gene: 13982 Mouse
- Entrez Gene: 397435 Pig
- Entrez Gene: 24890 Rat
- Omim: 133430 Human
- SwissProt: P49884 Cow
see all -
別名
- 7*/654 isoform antibody
- 7*/819 2 isoform antibody
- 7*/822 isoform antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)IHC image of Estrogen Receptor alpha staining in human breast ductal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with ab3575, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Estrogen Receptor alpha antibody (ab3575)ab3575 at a dilution of 5 µg/ml staining ER alpha in rat prostate tissue sections.
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Western blot - Anti-Estrogen Receptor alpha antibody (ab3575)All lanes : Anti-Estrogen Receptor alpha antibody (ab3575) at 1/1000 dilution
Lane 1 : Recombinant full-length human Estrogen Receptor alpha protein, 250 ng
Lane 2 : Recombinant full-length human Estrogen Receptor alpha protein, 125 ng
Lane 3 : Recombinant full-length human Estrogen Receptor alpha protein, 62.5 ng
Lane 4 : Recombinant full-length human Estrogen Receptor alpha protein, 31.25 ng
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 68 kDaBlocked with 5% milk in TBST for at least 1 hour at room temperature.
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Immunocytochemistry/ Immunofluorescence - Anti-Estrogen Receptor alpha antibody (ab3575)4% paraformaldehyde fixed 0.1% Triton permeabilized T-47D (human ductal breast epithelial tumor cell line) and negative control line Hs 578T cells stained for Estrogen Receptor alpha (green) using ab3575 (1/2000) in ICC/IF. DyLight 488-conjugated goat anti-rabbit IgG at 1/500.
参考文献
ab3575 は 12 報の論文で使用されています。
- Niranjan MK & Srivastava R Expression of estrogen receptor alpha in developing brain, ovary and shell gland of Gallus gallus domesticus: Impact of stress and estrogen. Steroids 146:21-33 (2019). PubMed: 30885650
- Glont SE et al. Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha. PLoS One 14:e0215340 (2019). PubMed: 30970003
- Wright KD et al. The p52 isoform of SHC1 is a key driver of breast cancer initiation. Breast Cancer Res 21:74 (2019). IHC . PubMed: 31202267
- Kim CS et al. Tamoxifen ameliorates obstructive nephropathy through Src and the PI3K/Akt/mTOR pathway. Biol Cell 111:18-27 (2019). PubMed: 30304547
- Tian X & Zhang Z miR-191/DAB2 axis regulates the tumorigenicity of estrogen receptor-positive breast cancer. IUBMB Life 70:71-80 (2018). PubMed: 29247596
