The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Predicted molecular weight: 160 kDa.
Use at an assay dependent concentration. PubMed: 24554440
Telomerase-binding protein EST1A is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It has been shown to promote the ability of TERT to elongate telomeres and may have some endonuclease function.
The predicted Mw for Human Telomerase-binding protein EST1A is 160 kDa. We are unsure why we are seeing the banding pattern at kDa however Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
ICC/IF image of ab87539 stained MCF7 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab87539, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 100% methanol fixed (5 min) MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data
Huang L et al. Antisense suppression of the nonsense mediated decay factor Upf3b as a potential treatment for diseases caused by nonsense mutations. Genome Biol19:4 (2018).
Read more (PubMed: 29334995) »
Muller M & Glaunsinger BA Nuclease escape elements protect messenger RNA against cleavage by multiple viral endonucleases. PLoS Pathog13:e1006593 (2017).
Read more (PubMed: 28841715) »