Anti-ERp57 抗体 [EPR10679(B)] (ab154197)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10679(B)] to ERp57
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ERp57 antibody [EPR10679(B)]
ERp57 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR10679(B)] to ERp57 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt,ICC/IF or IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HeLa, HepG2, Daudi and 293T cell lysates; Human pancreas and stomach tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR10679(B) -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154197の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Predicted molecular weight: 57 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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組織特異性
Detected in the flagellum and head region of spermatozoa (at protein level). -
配列類似性
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
細胞内局在
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 2923 Human
- Omim: 602046 Human
- SwissProt: P30101 Human
- Unigene: 591095 Human
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別名
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
see all
画像
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: ERp57 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HepG2 whole cell lysate (20 µg)
Lane 4: Hek293 whole cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab154197 observed at 57 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab154197 was shown to specifically react with ERp57 in wild-type HAP1 cells as signal was lost in ERp57 knockout cells. Wild-type and ERp57 knockout samples were subjected to SDS-PAGE. ab154197 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling ERp57 with ab154197 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-ERp57 antibody [EPR10679(B)] (ab154197) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 57 kDa -
Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling ERp57 with ab154197 at 1/50 dilution
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human endometrial carcinoma tissue using ab154197 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab154197 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab154197 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab154197 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab154197 は論文での使用が確認できていません。