Anti-ERp57 抗体 (ab13507)
Key features and details
- Rabbit polyclonal to ERp57
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ERp57 antibody
ERp57 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ERp57 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human, Chinese hamster -
免疫原
Tissue, cells or virus corresponding to Rat ERp57.
Database link: P11598 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13507の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
1/1000. Predicted molecular weight: 57 kDa. This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis.
|
| ICC/IF | (3) |
1/1000.
|
| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 57 kDa. This dilution detects Erp57 in 20µg of rat liver microsomal preparation by ECL analysis. |
|
ICC/IF
1/1000. |
ターゲット情報
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組織特異性
Detected in the flagellum and head region of spermatozoa (at protein level). -
配列類似性
Belongs to the protein disulfide isomerase family.
Contains 2 thioredoxin domains. -
細胞内局在
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 100689343 Chinese hamster
- Entrez Gene: 2923 Human
- Entrez Gene: 14827 Mouse
- Entrez Gene: 29468 Rat
- Omim: 602046 Human
- SwissProt: P30101 Human
- SwissProt: P27773 Mouse
- SwissProt: P11598 Rat
see all -
別名
- 58 kDa glucose regulated protein antibody
- 58 kDa glucose-regulated protein antibody
- 58 kDa microsomal protein antibody
see all
画像
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Western blot - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen PetrosyanAnti-ERp57 antibody (ab13507) at 1/2000 dilution + Human LNCaP whole cell lysate at 25 µg
Secondary
HRP-conjugated Donkey anti-rabbit at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Exposure time: 45 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)This image is courtesy of an Abreview submitted by Armen Petrosyanab13507 staining ERp57 in the Human PC-3 prostate cancer cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 1% serum for 1 hour at 22°C. Samples were incubated with primary antibody (1/50 in 1% Donkey serum in PBST) for 1 hour at 22°C. An undiluted Alexa Fluor® 594-conjugated Donkey anti-rabbit IgG polyclonal was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-ERp57 antibody (ab13507)ICC/IF image of ab13507 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13507, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-ERp57 antibody (ab13507)All lanes : Anti-ERp57 antibody (ab13507) at 1/1000 dilution
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from CHO-K1 cells
Lane 3 : Cell lysates prepared from human liver microsomes
Lane 4 : Cell lysates prepared from rat liver microsomes
Lane 5 : Cell lysates prepared from mouse liver microsomes
Predicted band size: 57 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (9)
ab13507 は 9 報の論文で使用されています。
- Lea RG et al. The fetal ovary exhibits temporal sensitivity to a 'real-life' mixture of environmental chemicals. Sci Rep 6:22279 (2016). IHC . PubMed: 26931299
- Petrosyan A et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem 290:6256-69 (2015). PubMed: 25605727
- Zhao Q et al. Discovery of porcine maternal factors related to nuclear reprogramming and early embryo development by proteomic analysis. Proteome Sci 13:18 (2015). WB . PubMed: 26155198
- Petrosyan A et al. Restoration of compact Golgi morphology in advanced prostate cancer enhances susceptibility to galectin-1-induced apoptosis by modifying mucin O-glycan synthesis. Mol Cancer Res 12:1704-16 (2014). PubMed: 25086069
- Li J et al. Identification and characterization of an oocyte factor required for sperm decondensation in pig. Reproduction 148:367-75 (2014). PubMed: 25030891
- Al-Gubory KH et al. Proteomic analysis of the sheep caruncular and intercaruncular endometrium reveals changes in functional proteins crucial for the establishment of pregnancy. Reproduction 147:599-614 (2014). WB, IHC-P ; Sheep . PubMed: 24446454
- Yu Y et al. Microinjection Manipulation Resulted in the Increased Apoptosis of Spermatocytes in Testes from Intracytoplasmic Sperm Injection (ICSI) Derived Mice. PLoS One 6:e22172 (2011). IHC-P, WB ; Mouse . PubMed: 21799787
- Ding Y et al. Proteomic analysis of colonic mucosa in a rat model of irritable bowel syndrome. Proteomics 10:2620-2630 (2010). WB ; Rat . PubMed: 20461717
- Atkin JD et al. Induction of the unfolded protein response in familial amyotrophic lateral sclerosis and association of protein-disulfide isomerase with superoxide dismutase 1. J Biol Chem 281:30152-65 (2006). PubMed: 16847061
