Application
Western blot
Sample
Mouse Tissue lysate - whole (Retinas)
Gel Running Conditions
Non-reduced Denaturing (TGX gel)
Loading amount
10 µg
Specification
Retinas
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2500
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 29, ~65 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
PROF. Minzhong Yu
Verified customer
投稿 May 09 2016