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Anti-ERM / Etv5 抗体 (ab102010)

Reviews (6) Submit a question References (18)
Western blot - Anti-ERM / Etv5 antibody (ab102010)
  • Western blot - Anti-ERM / Etv5 antibody (ab102010)
  • Immunocytochemistry - Anti-ERM / Etv5 antibody (ab102010)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)
  • Western blot - Anti-ERM / Etv5 antibody (ab102010)

Key features and details

  • Rabbit polyclonal to ERM / Etv5
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-ERM / Etv5 antibody
    ERM / Etv5 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to ERM / Etv5

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: ICC, WB, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Recombinant fragment corresponding to Human ERM/ Etv5 aa 244-404.
    Database link: P41161

  • ポジティブ･コントロール

    • WB: PC-3, A549 cell lysate

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab102010の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
1/100 - 1/1000.
WB (2)
1/500 - 1/10000. Predicted molecular weight: 58 kDa.

Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature.
IHC-P (2)
1/100 - 1/1000.

Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) is recommended.
特記事項
ICC
1/100 - 1/1000.
WB
1/500 - 1/10000. Predicted molecular weight: 58 kDa.

Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature.
IHC-P
1/100 - 1/1000.

Antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0) is recommended.

ターゲット情報

画像

  • Western blot - Anti-ERM / Etv5 antibody (ab102010)
    Western blot - Anti-ERM / Etv5 antibody (ab102010)
    All lanes : Anti-ERM / Etv5 antibody (ab102010) at 1/500 dilution

    Lane 1 : PC-3 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 58 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab102010 observed at 58 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab102010 was shown to react with ERM / Etv5 in Western blot. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab102010 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 500 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-ERM / Etv5 antibody (ab102010)
    Western blot - Anti-ERM / Etv5 antibody (ab102010)
    All lanes : Anti-ERM / Etv5 antibody (ab102010) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T whole cell extracts
    Lane 2 : Transfected HEK-293T whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 58 kDa



    10% SDS-PAGE

  • Immunocytochemistry - Anti-ERM / Etv5 antibody (ab102010)
    Immunocytochemistry - Anti-ERM / Etv5 antibody (ab102010)

    ab102010 staining ERM / Etv5 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody (1/500) and cytoskeleton stained red with Phalloidin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)

    ab102010 staining ERM / Etv5 in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody (1/500).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERM / Etv5 antibody (ab102010)
    Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using ab102010 at 1/100 dilution.
  • Western blot - Anti-ERM / Etv5 antibody (ab102010)
    Western blot - Anti-ERM / Etv5 antibody (ab102010)
    Anti-ERM / Etv5 antibody (ab102010) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE

参考文献 (18)

ab102010 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab102010 は 18 報の論文で使用されています。

  • Peng Y  et al. The role of E26 transformation-specific variant transcription factor 5 in colorectal cancer cell proliferation and cell cycle progression. Cell Death Dis 12:427 (2021). PubMed: 33931578
  • Zhang X  et al. Establishment of Etv5 gene knockout mice as a recipient model for spermatogonial stem cell transplantation. Biol Open 10:N/A (2021). PubMed: 33298570
  • Lin H  et al. IP6-assisted CSN-COP1 competition regulates a CRL4-ETV5 proteolytic checkpoint to safeguard glucose-induced insulin secretion. Nat Commun 12:2461 (2021). PubMed: 33911083
  • Li Z  et al. CRISPR Screens Identify Essential Cell Growth Mediators in BRAF Inhibitor-resistant Melanoma. Genomics Proteomics Bioinformatics 18:26-40 (2020). PubMed: 32413516
  • Feng H  et al. Targeting tumor cell-derived CCL2 as a strategy to overcome Bevacizumab resistance in ETV5+ colorectal cancer. Cell Death Dis 11:916 (2020). PubMed: 33099574
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-ERM / Etv5 antibody

 Poor
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Insulin secreting beta cell derived line)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Treatment
Transfected with siRNA ETV5
Specification
Insulin secreting beta cell derived line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. VICENTA CAZARES

Verified customer

投稿 Mar 08 2022

ChIP abreview for Anti-ERM / Etv5 antibody

 Good
Abreviews
abreview image
Application
ChIP
Sample
Mouse Cell lysate - nuclear (pancreatic tumor cells)
Specification
pancreatic tumor cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 01 2017

Western blot abreview for Anti-ERM / Etv5 antibody

 Below Average
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (pancreatic tumor cells)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
50 µg
Specification
pancreatic tumor cells
Blocking step
odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 01 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ERM / Etv5 antibody

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (Prepubertal testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
No
Specification
Prepubertal testis
Blocking step
5% serum + 3% BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 01 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ERM / Etv5 antibody

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse Pancreatic Tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Mouse Pancreatic Tumor
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 31 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-ERM / Etv5 antibody

 Good
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spermatogonia)
Specification
Spermatogonia
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton/PBS
Blocking step
Roche blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 10 2013

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