Anti-Erlin-2 抗体 [EPR8088] (ab129207)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8088] to Erlin-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Erlin-2 antibody [EPR8088]
Erlin-2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR8088] to Erlin-2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human Erlin-2 aa 50-150. The exact sequence is proprietary.
-
ポジティブ・コントロール
- Fetal kidney, 293T, HepG2, and HeLa lysates; Human endometrium adenocarcinoma; HepG2 cells
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 5.40 x 10 -13 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR8088 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129207の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
|
|
IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/100 - 1/250.
|
特記事項 |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 38 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
ターゲット情報
-
機能
Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Also involved in ITPR1 degradation by the ERAD pathway. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the band 7/mec-2 family. -
細胞内局在
Endoplasmic reticulum membrane. Associated with lipid raft-like domains of the endoplasmic reticulum membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 11160 Human
- Entrez Gene: 244373 Mouse
- Omim: 611605 Human
- SwissProt: O94905 Human
- SwissProt: Q8BFZ9 Mouse
- Unigene: 705490 Human
- Unigene: 277699 Mouse
-
別名
- C8orf2 antibody
- Chromosome 8 open reading frame 2 antibody
- Endoplasmic reticulum lipid raft associated protein 2 antibody
see all
画像
-
All lanes : Anti-Erlin-2 antibody [EPR8088] (ab129207) at 1/1000 dilution
Lane 1 : Fetal kidney tissue lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 38 kDa -
ab129207, at 1/50 dilution, staining Erlin-2 in paraffin-embedded Human endometrium adenocarcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
ab129207, at 1/100 dilution, staining Erlin-2 in HepG2 cells by Immunofluorescence.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (3)
ab129207 は 3 報の論文で使用されています。
- Agrawal RR et al. Alzheimer's-Associated Upregulation of Mitochondria-Associated ER Membranes After Traumatic Brain Injury. Cell Mol Neurobiol N/A:N/A (2022). PubMed: 36571634
- Rossi AM et al. Quantal Ca2+ release mediated by very few IP3 receptors that rapidly inactivate allows graded responses to IP3. Cell Rep 37:109932 (2021). PubMed: 34731613
- Liu Y et al. Molecular and Immune Characteristics for Lung Adenocarcinoma Patients With ERLIN2 Overexpression. Front Immunol 11:568440 (2020). PubMed: 33424830