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  1. Link

    erk1-pt202py204--erk2-pt185py187-antibody-ab4819.pdf

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Neuroscience Neurotransmission Intracellular Signaling Kinases
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Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 抗体 (ab4819)

  • Datasheet
Reviews (4)Q&A (7)References (30)

Product price, shipping and contact information

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)

Key features and details

  • Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-ERK1 antibody [Y72] (ab32537)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
    Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human Erk1 (pT202/pY204) + Erk2 (pT185/pY187). This region is conserved among many species including rat, mouse, cow, frog, snail, nematode, and fruit fly.
    (Peptide available as ab5313, ab5354, ab5255)

  • ポジティブ・コントロール

    • WB: MDA-MB-231, U-87 MG, Sh-SY5Y, HeLa, PC-12 whole cell lysates, MDA-MB-231 whole cell lysate with treatment of EGF(100 ng/mL for 15 mins. IHC-P: Human breast and colon carcinoma, mouse stomach tissue.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.1% BSA

    BSA is IgG and protease free
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK 1 + 2. The final product is generated by affinity chromatography using an ERK 1 + 2-derived peptide that is phosphorylated at threonine 202/185 and tyrosine 204/187, respectively, within the activation loop.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Immunology
    • Innate Immunity
    • Cytokines
    • Interleukins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Focal Adhesions
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Phosphatases
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4819の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (4)
1/1000. Predicted molecular weight: 44,42 kDa.
IHC-P
1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000. Predicted molecular weight: 44,42 kDa.
IHC-P
1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus with ab4819 at 1/50 dilution, compared to a negative control without primary antibody (left).

    To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution

    Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate, with treatment of EGF(100 ng/mL for 15 mins)
    Lane 2 : MDA-MB-231 whole cell lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 44,42 kDa



    Bands of 42 kDa and 44 kDa corresponding to Phospho-p44 MAPK + p42 MAPK pThr185 + pTyr187 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus withab4819 at 1/20 dilution, compared to a negative control without primary antibody (left).

    To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus with ab4819 at 1/20 dilution, compared to a negative control without primary antibody (left).

    To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1/20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate, unstimulated
    Lanes 2-6 : PC-12 whole cell lysate, stimulated with 0.5 M sorbitol for 5 minutes

    Secondary
    All lanes : Goat F (ab')2 anti-rabbit IgG HRP conjugate

    Predicted band size: 44,42 kDa



    Extracts of PC12 cells were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.

    The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, and then incubated with ab4819 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with:

    Lane 1 and 2: no peptide

    Lane 3: the non-phosphopeptide corresponding to the phosphopeptide immunogen

    Lane 4: a generic phosphothreonine-containing peptide

    Lane 5: a generic phosphotyrosine-containing peptide

    Labe 6: the phosphopeptide immunogen 

    Detection: Pierce SuperSignal™ method.

  • Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)
    All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution

    Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : NIH/3T3 whole cell lysate, treated with either PDGF

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-rabbit secondary antibody conjugated to Alexa fluor 680

    Predicted band size: 44,42 kDa



    Data was analyzed on the LI-COR Odyssey® Infrared Imaging System. 

プロトコール

  • Peptide Competition Experiment
  • Western blotting protocol

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (30)

ab4819 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4819 は 30 報の論文で使用されています。

  • Bae Y  et al. miR-30c-1 encourages human corneal endothelial cells to regenerate through ameliorating senescence. Aging (Albany NY) 13:9348-9372 (2021). PubMed: 33744867
  • Jung HY  et al. NOX1 Inhibition Attenuates Kidney Ischemia-Reperfusion Injury via Inhibition of ROS-Mediated ERK Signaling. Int J Mol Sci 21:N/A (2020). PubMed: 32967113
  • Semina EV  et al. Downregulation of uPAR promotes urokinase translocation into the nucleus and epithelial to mesenchymal transition in neuroblastoma. J Cell Physiol 235:6268-6286 (2020). PubMed: 31990070
  • Joo HJ  et al. SIRT1 Activation Using CRISPR/dCas9 Promotes Regeneration of Human Corneal Endothelial Cells through Inhibiting Senescence. Antioxidants (Basel) 9:N/A (2020). PubMed: 33158256
  • Novoseletskaya E  et al. Mesenchymal Stromal Cell-Produced Components of Extracellular Matrix Potentiate Multipotent Stem Cell Response to Differentiation Stimuli. Front Cell Dev Biol 8:555378 (2020). PubMed: 33072743
View all Publications for this product

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1-10 of 11 Abreviews or Q&A

Western blot abreview for Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10000 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Specification
Mouse Neuroblastoma (Neuro2A)
Treatment
Synthetic Spiro analogues
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 12 2014

Western blot abreview for Anti-ERK1 + ERK2 (phospho T185 + T202 + Y204 + Y187) antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Culex pipiens Tissue lysate - whole (whole fly homogenates)
Specification
whole fly homogenates
Treatment
30 minutes after feeding with sheep blood
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Jiawen Xu

Verified customer

投稿 Aug 20 2013

Western blot abreview for Anti-ERK1 + ERK2 (phospho T185 + T202 + Y204 + Y187) antibody

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Termite Tissue lysate - whole (termite)
Loading amount
40 µg
Specification
termite
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 6% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Chris Mattison

Verified customer

投稿 Apr 09 2012

Western blot abreview for Anti-ERK1 + ERK2 (phospho T185 + T202 + Y204 + Y187) antibody

Poor
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (prostate)
Loading amount
25 µg
Specification
prostate
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jan 03 2007

Question

WB with HEK293
sees high background all over the membrane
loaded 7-20 ug
Ab: 1/2000 2h RT
block: 5% BSA o/n 4 degree
2nd: OK
followed WB protocol on datasheet
blocking with 5% milk gives no background and no bands (loaded only 5 ug total protein)

Read More

Abcam community

Verified customer

Asked on Aug 23 2012

Answer

Thank you for contacting us.

I am sorry to that the antibody seems not tobe working as expected.

As discussed over the phone, please send me some of your WB images and additional protocol information, so that I can pass this on to the lab for additional suggestions and feedback.

I look forward to hear back from you and assist you further.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Aug 23 2012

Question



It is a great pleasure that I could directlytalk to you. Thank you very much for your response. I do appreciate your consideration andmangement.

Read More

Abcam community

Verified customer

Asked on Jun 18 2012

Answer

I am happy to help! Please feel free to contact me if you need anything further.

Read More

Abcam Scientific Support

Answered on Jun 18 2012

Question

I have aliquoted this vial of ab4819 into 5ul aliquots and only been able to fill 8 tubes where I should have been able to fill 10. I am missing about 10ul.

Read More

Abcam community

Verified customer

Asked on Jun 15 2012

Answer

I am sorry this product did not contain the volume as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Abcam Scientific Support

Answered on Jun 15 2012

Question

send new discount code (test in termites) for ab4819

Read More

Abcam community

Verified customer

Asked on Apr 27 2012

Answer

Thank you for your phone call.


DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab4819 in termites. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for termitesand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.


The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Apr 27 2012

Question

I would like to test ab4819 for use on termite samples.

Read More

Abcam community

Verified customer

Asked on Nov 30 2011

Answer

DISCOUNT CODE: *** Expiration date: March 30th, 2012 I am very pleased to hear you would like to accept our offer and test ab4819 in termite. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for termite and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.  

Read More

Abcam Scientific Support

Answered on Nov 30 2011

Question

Hi, I was wondering what is the difference between AB 16869 and AB 4819. They both say they are phoso erk 1 and 2. Thank you

Read More

Abcam community

Verified customer

Asked on Jul 21 2006

Answer

Thank you for contacting us with your query. There is only a small difference between these two antibodies if you are going to make a choice between the two. They will both recognise phosphorylated ERK1 and ERK2. ERK1 and ERK2 are phosphorylated within the activation loop on both a Threonine and a Tyrosine residue (within a Thr-Glu-Tyr motif) by MEKs (MAPK/ERK kinases), thereby greatly elevating their activity . Ab4819 recognises phosphorylation on the T185 and T202 residues Ab16869 recognises phosphorylation on T202/T183 and Y204/185 residues. These phosphorylated residues are common to ERK 1 and 2. Both the antibodies are raised against synthetic peptide. I hope this helps! Please get back to me if there is anything else I can help you with. With thanks.

Read More

Abcam Scientific Support

Answered on Jul 21 2006

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