Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 抗体 (ab4819)
Key features and details
- Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody
Erk1 (pT202/pY204) + Erk2 (pT185/pY187) 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Erk1 (pT202/pY204) + Erk2 (pT185/pY187) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
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ポジティブ･コントロール
- WB: MDA-MB-231, U-87 MG, Sh-SY5Y, HeLa, PC-12 whole cell lysates, MDA-MB-231 whole cell lysate with treatment of EGF(100 ng/mL for 15 mins. IHC-P: Human breast and colon carcinoma, mouse stomach tissue.
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特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA
BSA is IgG and protease free -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the sites of phosphorylation to remove antibody that is reactive with non-phosphorylated ERK 1 + 2. The final product is generated by affinity chromatography using an ERK 1 + 2-derived peptide that is phosphorylated at threonine 202/185 and tyrosine 204/187, respectively, within the activation loop. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab4819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 44,42 kDa. | |
| IHC-P | 1/10 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus with ab4819 at 1/50 dilution, compared to a negative control without primary antibody (left).
To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution
Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate, with treatment of EGF(100 ng/mL for 15 mins)
Lane 2 : MDA-MB-231 whole cell lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 5 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 44,42 kDaBands of 42 kDa and 44 kDa corresponding to Phospho-p44 MAPK + p42 MAPK pThr185 + pTyr187 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus withab4819 at 1/20 dilution, compared to a negative control without primary antibody (left).
To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)Immunohistochemical analysis of paraffin-embedded mouse stomach tissue (right) labeling ERK1/2 (pTpY185/187) in the cytoplasm and nucleus with ab4819 at 1/20 dilution, compared to a negative control without primary antibody (left).
To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab4819 diluted in 3% BSA-PBS at a dilution of 1/20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate, unstimulated
Lanes 2-6 : PC-12 whole cell lysate, stimulated with 0.5 M sorbitol for 5 minutes
Secondary
All lanes : Goat F (ab')2 anti-rabbit IgG HRP conjugate
Predicted band size: 44,42 kDaExtracts of PC12 cells were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF.
The membrane was blocked with a 5% BSA-TBST buffer overnight at 4°C, and then incubated with ab4819 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with:
Lane 1 and 2: no peptide
Lane 3: the non-phosphopeptide corresponding to the phosphopeptide immunogen
Lane 4: a generic phosphothreonine-containing peptide
Lane 5: a generic phosphotyrosine-containing peptide
Labe 6: the phosphopeptide immunogen
Detection: Pierce SuperSignal™ method.
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Western blot - Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819)All lanes : Anti-Erk1 (pT202/pY204) + Erk2 (pT185/pY187) antibody (ab4819) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate, treated with either PDGF
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-rabbit secondary antibody conjugated to Alexa fluor 680
Predicted band size: 44,42 kDaData was analyzed on the LI-COR Odyssey® Infrared Imaging System.
プロトコール
データシートおよび資料
参考文献 (24)
ab4819 は 24 報の論文で使用されています。
- Li W et al. Berberine suppresses IL-33-induced inflammatory responses in mast cells by inactivating NF-?B and p38 signaling. Int Immunopharmacol 66:82-90 (2019). PubMed: 30445310
- Ursoli Ferreira F et al. Endothelial Cells Tissue-Specific Origins Affects Their Responsiveness to TGF-ß2 during Endothelial-to-Mesenchymal Transition. Int J Mol Sci 20:N/A (2019). PubMed: 30678183
- Trivett MT et al. Preferential Small Intestine Homing and Persistence of CD8 T Cells in Rhesus Macaques Achieved by Molecularly Engineered Expression of CCR9 and Reduced Ex Vivo Manipulation. J Virol 93:N/A (2019). PubMed: 31434738
- Fa Z et al. MicroRNA-150 suppresses the growth and malignant behavior of papillary thyroid carcinoma cells via downregulation of MUC4. Exp Ther Med 16:45-52 (2018). PubMed: 29896226
- Kole D et al. High molecular weight FGF2 isoforms demonstrate canonical receptor-mediated activity and support human embryonic stem cell self-renewal. Stem Cell Res 21:106-116 (2017). PubMed: 28433654
