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  1. Link

    erk1--erk2-antibody-ab17942.pdf

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Anti-ERK1 + ERK2 抗体 (ab17942)

  • Datasheet
  • SDS
Reviews (25)Q&A (3)References (279)

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保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
  • Immunocytochemistry - Anti-ERK1 + ERK2 antibody (ab17942)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
  • Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
  • Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
  • Western blot - Anti-ERK1 + ERK2 antibody (ab17942)

Key features and details

  • Rabbit polyclonal to ERK1 + ERK2
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-ERK1 + ERK2 antibody [EPR17526] (ab184699)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-ERK1 + ERK2 antibody
    ERK1 + ERK2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to ERK1 + ERK2
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, IHC-P, WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human ERK1 + ERK2 aa 317-339 (C terminal).
    Sequence:

    RIT VEEALAHPYL EQYYDPTDE


    Database link: P27361
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Please note that this is an intracellular epitope.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.1% BSA

    phosphate buffered saline without Mg2+ and
    Ca2+.
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Tangles & Tau
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • MAPK Pathway
    • Stem Cells
    • Signaling Pathways
    • TGF beta
    • Cytoplasmic
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Cell adhesion
    • Other
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Other
    • Cancer
    • Cell Death
    • Apoptosis
    • Other

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Mouse kidney tissue lysate - total protein (0 days) (ab7261)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab17942の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC (1)
Use a concentration of 1 µg/ml.
IHC-P (2)
1/10 - 1/100.
WB (15)
1/1000. Predicted molecular weight: 42-44 kDa.
特記事項
ICC
Use a concentration of 1 µg/ml.
IHC-P
1/10 - 1/100.
WB
1/1000. Predicted molecular weight: 42-44 kDa.

ターゲット情報

  • 機能

    Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
    Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.
  • 配列類似性

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • ドメイン

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • 翻訳後修飾

    Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession P28482 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5594 Human
    • Entrez Gene: 5595 Human
    • Entrez Gene: 26413 Mouse
    • Entrez Gene: 26417 Mouse
    • Entrez Gene: 116590 Rat
    • Entrez Gene: 50689 Rat
    • Omim: 176948 Human
    • Omim: 601795 Human
    • SwissProt: P27361 Human
    • SwissProt: P28482 Human
    • SwissProt: P63085 Mouse
    • SwissProt: Q63844 Mouse
    • SwissProt: P21708 Rat
    • SwissProt: P63086 Rat
    • Unigene: 431850 Human
    • Unigene: 196581 Mouse
    • Unigene: 34914 Rat
    see all
  • 別名

    • ERK 1 antibody
    • ERK 2 antibody
    • ERK-1 antibody
    • ERK-2 antibody
    • ERK1 antibody
    • erk1/2 antibody
    • ERK2 antibody
    • ERT1 antibody
    • ERT2 antibody
    • Extracellular signal regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 1 antibody
    • Extracellular signal-regulated kinase 2 antibody
    • HS44KDAP antibody
    • HUMKER1A antibody
    • Insulin-stimulated MAP2 kinase antibody
    • MAP kinase 1 antibody
    • MAP kinase 2 antibody
    • MAP kinase 3 antibody
    • MAP kinase isoform p42 antibody
    • MAP kinase isoform p44 antibody
    • MAPK 1 antibody
    • MAPK 2 antibody
    • MAPK 3 antibody
    • Mapk1 antibody
    • MAPK2 antibody
    • MAPK3 antibody
    • Microtubule-associated protein 2 kinase antibody
    • Mitogen-activated protein kinase 1 antibody
    • Mitogen-activated protein kinase 2 antibody
    • Mitogen-activated protein kinase 3 antibody
    • MK01_HUMAN antibody
    • p38 antibody
    • p40 antibody
    • p41 antibody
    • p41mapk antibody
    • p42-MAPK antibody
    • P42MAPK antibody
    • p44-ERK1 antibody
    • p44-MAPK antibody
    • p44ERK1 antibody
    • p44MAPK antibody
    • PRKM 2 antibody
    • PRKM1 antibody
    • PRKM2 antibody
    • PRKM3 antibody
    • protein tyrosine kinase ERK2 antibody
    see all

画像

  • Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
    Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
    All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution

    Lane 1 : K562 cells
    Lane 2 : Daudi cells
    Lane 3 : Hep G2 cells
    Lane 4 : PC-3 cells
    Lane 5 : NIH 3T3 cells

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG - HRP Secondary Antibody at 1/5000 dilution

    Predicted band size: 42-44 kDa

  • Immunocytochemistry - Anti-ERK1 + ERK2 antibody (ab17942)
    Immunocytochemistry - Anti-ERK1 + ERK2 antibody (ab17942)

    Immunofluorescent analysis of ERK1 + ERK2 Antibody was done on 70% confluent log phase U87-MG cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ab17942 at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin. Panel d is a merged image showing cytoplasmic and nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
    Immunohistochemistry analysis of ERK1/2 (pan) showing staining in the cytoplasm and nucleus of paraffin-embedded mouse stomach tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab17942 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
     
     
  • Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
    Western blot - Anti-ERK1 + ERK2 antibody (ab17942)

    Western Blot for ab17942.

    Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated
    with goat anti-rabbit IgG alkaline phosphatase.

    These data show that ab17942 ERK1&2 antibody allows the total amount of ERK1&2 to be measured.

     

    Extracts prepared from PC12 cells not stimulated (-), or stimulated with NGF (+) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4C, then were incubated with ERK1&2 pan antibody for two hours at room temperature in a 3% BSA-TBST buffer. After washing, membranes were incubated with goat anti-ra

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)

    Immunohistochemistry analysis of ERK1/2 (pan) showing staining in the cytoplasm and nucleus of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab17942 diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ERK1 + ERK2 antibody (ab17942)

    Immunohistochemistry analysis of ERK1/2 (pan) showing staining in the cytoplasm and nucleus of paraffin-embedded human colon carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab17942 diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
    Western blot - Anti-ERK1 + ERK2 antibody (ab17942)This image is courtesy of an anonymous Abreview
    All lanes : Anti-ERK1 + ERK2 antibody (ab17942) at 1/1000 dilution

    Lane 1 : Rat spinal cord tissue homogenate from animals that underwent Sham surgery
    Lanes 2-3 : Rat spinal cord tissue homogenate from animals that underwent L5 nerve transection

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit antibody at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 42-44 kDa
    Observed band size: 42,44 kDa why is the actual band size different from the predicted?


    Exposure time: 5 minutes


    The tissue was harvested seven days post surgery, sonicated with RIPA buffer and the protein estimate made by Lowry. A 10% SDS-PAGE gel was run for 1.5 hr at 100V and transfered to PVDF membrane for 1.5 hr at 274 mA. The blot was blocked with 5% BSA for 1 hour at 23°C. The primary antibody was incubated with the blot for 18 hours at 4°C.

    See Abreview

  • Western blot - Anti-ERK1 + ERK2 antibody (ab17942)
    Western blot - Anti-ERK1 + ERK2 antibody (ab17942)Image from PLoS One. 2014; 9(6): e99219. Fig3A, doi: 10.1371/journal.pone.0099219 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Western blot analysis of Mice retinas (40-50μg/lane) labelling with anti-ERK1/2 at 1:300 (ab17942) and mouse monoclonal anti-phosphorylated ERK1/2 at 1:300 (ab50011), in 5% nonfat milk in TBST overnight at 4ºC. HRP conjugated antibodies were used as the secondary antibodies.

    Data is expressed as percentage change in phosphorylated ERK1/2 (p-ERK1/2) over total ERK1/2 (t-ERK1/2) calculated in control and diabetic mice maintained with and without Edaravone treatment

    Results are expressed as mean±SD. Values obtained from Normal group are considered as 100%. *P<0.05, ***P<0.001 vs. Normal, #P<0.05 vs. Edaravone

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (279)

ab17942 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab17942 は 279 報の論文で使用されています。

  • Ren C  et al. SHCBP1 Promotes the Progression of Esophageal Squamous Cell Carcinoma Via the TGFß Pathway. Appl Immunohistochem Mol Morphol 29:136-143 (2021). PubMed: 32769441
  • Wang YY  et al. Nogo-A aggravates oxidative damage in oligodendrocytes. Neural Regen Res 16:179-185 (2021). PubMed: 32788474
  • Zhang Z  et al. Batroxobin inhibits astrocyte activation following nigrostriatal pathway injury. Neural Regen Res 16:721-726 (2021). PubMed: 33063734
  • Zhao X  et al. Glucocorticoids decreased Cx43 expression in osteonecrosis of femoral head: The effect on proliferation and osteogenic differentiation of rat BMSCs. J Cell Mol Med 25:484-498 (2021). PubMed: 33205619
  • Wang H  et al. Oncometabolite L-2-hydroxyglurate directly induces vasculogenic mimicry through PHLDB2 in renal cell carcinoma. Int J Cancer 148:1743-1755 (2021). PubMed: 33320958
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 28 Abreviews or Q&A

Western blot abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse embryonic Fibroblasts)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
Mouse embryonic Fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 23 2018

Western blot abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (293T)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Oct 23 2018

Western blot abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Total protein from zebrafish testicle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
Animals exposed to bisphenol A during 21 days
Specification
Total protein from zebrafish testicle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Aug 07 2018

Western blot abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampus)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Hippocampus
Blocking step
LiCOR blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 24°C
Read More

Abcam user community

Verified customer

投稿 Aug 27 2015

Immunohistochemistry (Frozen sections) abreview for Anti-ERK1 + ERK2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (retina)
Specification
retina
Permeabilization
Yes - triton x100
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 Feb 09 2015

Western blot abreview for Anti-ERK1 + ERK2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Culex pipiens Tissue lysate - whole (whole fly homogenates)
Specification
whole fly homogenates
Treatment
30 minutes after blood feeding with sheep blood
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Jiawen Xu

Verified customer

投稿 Aug 20 2013

Western blot abreview for Anti-ERK1 + ERK2 antibody

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (stomach cancer)
Loading amount
30 µg
Specification
stomach cancer
Treatment
10uM WIN 55,212-2 for 24hrs
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Apr 03 2013

Western blot abreview for Anti-ERK1 + ERK2 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2-OS)
Loading amount
25 µg
Specification
U2-OS
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Odyssey buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C
Read More

Abcam user community

Verified customer

投稿 Oct 18 2012

Question

Hola buenos días,
me pongo en contacto con ustedes para comentarles una duda sobre el anticuerpoanti-ERK1+ERK2 ANTIBODY (ab17942) de abcam. A diferencia de otros anticuerpos no especifica ni en la hoja correspondiente ni en el tubito, la concentración a la que está. Solo indica el volumen50 µl ( a pesar de que en la hoja de especificaciones indica este volumen como concentración). Muchas gracias por su atención. Un saludo,

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Abcam community

Verified customer

Asked on Apr 19 2012

Answer

Gracias por contactarnos.

La concentración de este anticuerpo es dependiente del lote. He conseguido localizar el único pedido que tenemos de la Univ. de XXXXX de este producto para comprobar el lote enviado, que si no me equivoco es XXXXXX


La concentración del mismo es 0.5 mg/mL.

Espero haberte ayudado. En caso contrario, no dudes en contactarnos de nuevo.

Read More

Abcam Scientific Support

Answered on Apr 19 2012

Question

Hello, I want an antibody for confocal that is specific for total ERK1 & 2. Is ab17942 the correct one? I also want an antibody specific for phosphorylated ERK1/2 and believe ab50011 is the correct one. The antibody for total ERK should not identify phosphoERK. Could you tell me if these are the correct antibodies.

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Abcam community

Verified customer

Asked on Oct 05 2011

Answer

For the detection of total ERK1+2 we recommend ab17942, a Rabbit polyclonal directed against amino acids 317-339 of Human ERK1+ERK2. This region is not subject to phosphorylation. ab17942 is covered by our Abpromise guarantee to work in ICC/IF, IHC-Fr, IHC-P, IP, WB and Human, Mouse, Rat, Cow, Dog. For the detection of the phosphorylated ERK1+2 we recommend ab50011, a Mouse monoclonal specific for the active, dually-phosphorylated form of MAP kinase (ERK-1 and ERK-2, 44 kDa and 42 kDa, respectively). It does not recognise the non-phosphorylated or the monophosphorylated forms of the MAP kinase molecule or the diphosphorylated forms of JUN kinase and p38 MAP kinase. The epitope recognized by ab50011 is located in the region of phosphorylated threonine and tyrosine residues within the regulatory site of active MAP kinase (Thr185 and Tyr187 in ERK-2). ab50011 is guaranteed to work in ELISA, ICC/IF, IHC-P, IP, WB and Human, Mouse, Rat, Cow, Drosophila, yeast. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Abcam Scientific Support

Answered on Oct 05 2011

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